| Full text | |
| Author(s): Show less - |
Candreva, Thamiris
[1]
;
Kuhl, Carolina M. C.
[1]
;
Burger, Beatriz
[1]
;
dos Anjos, Mariah B. P.
[1]
;
Torsoni, Marcio A.
[2]
;
Consonni, Silvio R.
[3]
;
Crisma, Amanda R.
[4]
;
Fisk, Helena L.
[5]
;
Calder, Philip C.
[6, 7, 5]
;
de Mato, Felipe C. P.
[8]
;
Sernaglia, Erica M.
[8]
;
Vinolo, Marco A. R.
[8]
;
Rodrigues, Hosana G.
[1]
Total Authors: 13
|
| Affiliation: | [1] Univ Estadual Campinas, Sch Appl Sci, Lab Nutrients & Tissue Repair, Limeira, SP - Brazil
[2] Univ Estadual Campinas, Sch Appl Sci, Lab Metab Disorders, Limeira, SP - Brazil
[3] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, Campinas, SP - Brazil
[4] Univ Sao Paulo, Dept Physiol & Biophys, Sao Paulo, SP - Brazil
[5] Univ Southampton, Human Dev & Hlth, Fac Med, Southampton, Hants - England
[6] Univ Southampton, Southampton, Hants - England
[7] Univ Hosp Southampton NHS Fdn Trust, NIHR Southampton Biomed Res Ctr, Southampton, Hants - England
[8] Univ Estadual Campinas, Inst Biol, Dept Genet Evolut Microbiol & Immunol, Campinas, SP - Brazil
Total Affiliations: 8
|
| Document type: | Journal article |
| Source: | Clinical Science; v. 133, n. 22, p. 2345-2360, NOV 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
There is no consensus on the effects of omega-3 (omega-3) fatty acids (FA) on cutaneous repair. To solve this problem, we used 2 different approaches: (1) FAT-1 transgenic mice, capable of producing endogenous omega-3 FA; (2) wild-type (WT) mice orally supplemented with DHA-enriched fish oil. FAT-1 mice had higher systemic (serum) and local (skin tissue) omega-3 FA levels, mainly docosahexaenoic acid (DHA), in comparison with WT mice. FAT-1 mice had increased myeloperoxidase (MPO) activity and content of CXCL-1 and CXCL-2, and reduced IL-10 in the skin wound tissue three days after the wound induction. Inflammation was maintained by an elevated TNF-alpha concentration and presence of inflammatory cells and edema. Neutrophils and macrophages, isolated from FAT-1 mice, also produced increased TNF-alpha and reduced IL-10 levels. In these mice, the wound closure was delayed, with a wound area 6-fold bigger in relation with WT group, on the last day of analysis (14 days post-wounding). This was associated with poor orientation of collagen fibers and structural aspects in repaired tissue. Similarly, DHA group had a delay during late inflammatory phase. This group had increased TNF-alpha content and CD45(+)F4/80(+) cells at the third day after skin wounding and increased concentrations of important metabolites derived from omega-3, like 18-HEPE, and reduced concentrations of those from omega-6 FA. In conclusion, elevated DHA content, achieved in both FAT-1 and DHA groups, slowed inflammation resolution and impaired the quality of healed skin tissue. (AU) | |
| FAPESP's process: | 14/15127-9 - Multiuser equipment approved in grant 2013/06810-4: flow citometry BD Accury |
| Grantee: | Hosana Gomes Rodrigues |
| Support Opportunities: | Multi-user Equipment Program |
| FAPESP's process: | 13/06810-4 - Mechanisms of action of omega-3 and omega-6 in the tissue repair process: neuro-immune focus |
| Grantee: | Hosana Gomes Rodrigues |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 16/02021-3 - Wound healing in FAT -1 mice: involvement of anti- inflammatory cholinergic pathway |
| Grantee: | Thamiris Candreva Robles |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 16/23298-3 - Analysis of phosphatidylcholine lipid fraction in fat-1 animals in the context of wound healing |
| Grantee: | Thamiris Candreva Robles |
| Support Opportunities: | Scholarships abroad - Research Internship - Master's degree |