Biogenic synthesis and antimicrobial activity of silica-coated silver nanoparticles for esthetic dental applications

Author(s)

Rodrigues, Marcela Charantola ^{[1, 2]} ; Rolim, Wallace Rosado ^[3] ; Viana, Marina Mariante ^[1] ; Souza, Thais Rodrigues ^[1] ; Goncalves, Flavia ^[4] ; Tanaka, Caio Junji ^[5] ; Bueno-Silva, Bruno ^[5] ; Seabra, Amedea Barozzi ^[3]

Affiliation

^[1] Cruzeiro Sul Univ UNICSUL, Postgrad Dept, Galvao Bueno St 868, BR-01506000 Sao Paulo, SP - Brazil

^[2] Municipal Univ Sao Caetano Sul USCS, Santo Antonio St 50, BR-09521160 Sao Caetano do Sul, SP - Brazil

^[3] Fed Univ ABC UFABC, Ctr Nat & Human Sci CCNH, Estados Ave 5001, BR-09210580 Santo Andre, SP - Brazil

^[4] Ibirapuera Univ UNIB, Interlagos Ave 1329, BR-04661100 Sao Paulo, SP - Brazil

^[5] Univ Guarulhos, Dent Res Div, Teresa Cristina Sq 229, BR-07023070 Guarulhos, SP - Brazil

• Document type: Journal article
• Source: Journal of Dentistry; v. 96, MAY 2020.
• Web of Science Citations: 0

Abstract

Objectives: this study aimed to synthesize AgNPs from green tea (GT) extract, forming GT-AgNPs, and to coat their surfaces with silica, resulting in light-colored Ag@SiO2 nanoparticles. Materials and Methods: particles were characterized and tested for minimal inhibitory concentration (MIC), biofilm formation against Streptococcus mutans and cytotoxicity evaluation on dental pulp fibroblasts. Results: X-ray diffraction (XRD) confirmed the formation of pure AgNPs, whereas energy dispersive X-ray spectroscopy (EDS) mapped their elemental atoms. Dynamic light scattering (DLS) demonstrated formation of particles at nanoscale, with moderate polydispersity and negative zeta potential, in agreement with nanoparticle tracking analysis (NTA) size measurements. Fourier-transformed infrared (FTIR) spectroscopy confirmed the successful condensation of silica, which significantly increased surface area by 50%, as assayed by surface area analysis (BET). Thermogravimetric analysis showed a 18%-mass of silica on the surface of Ag@SiO(2)NPs. Transmission electron microscopy (TEM) displayed the spherical shape of nanoparticles and average size of 11 nm for GT-AgNPs and Ag@SiO(2)NPs. Ag@SiO(2)NPs demonstrated potent antimicrobial action against S. mutans, with MIC determined as 600 mu g/mL, and inhibition of approximately 44% (p < 0.05) of biofilm formation. At the MIC concentrations, both NPs did not exhibit cytotoxicity. Conclusion: Ag@SiO(2)NPs might have a useful application in dental materials. Clinical significance: The possibility of incorporating antimicrobial properties in restorative materials without compromising esthetics makes the AgNPs@SiO2 NPs promising agents against S. mutans biofilm formation, hence the prevention of dental caries. This represents a great step towards the development of more interactive biomaterials in dentistry to overcome clinical problems. (AU)

• FAPESP's process: 14/50906-9 - INCT 2014: in Functional Complex Materials
• Grantee: Fernando Galembeck
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/22999-0 - Development of silver nanoparticles coated with silicon dioxide and their application in experimental dental composites
• Grantee: Marcela Charantola Rodrigues
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/08194-2 - Essential oil amended with metal nanoparticles functionalized with nitric oxide as a strategy to control plant pathogens in the agriculture
• Grantee: Amedea Barozzi Seabra
• Support Opportunities: Regular Research Grants