Antioxidant, antiproliferative and healing properties of araticum (Annona crassiflora Mart.) peel and seed

Author(s):Show less -

Prado, Livia Garcia ^[1] ; Arruda, Henrique Silvano ^[1] ; Peixoto Araujo, Nayara Macedo ^[1] ; de Oliveira Braga, Lucia Elaine ^{[2, 3]} ; Banzato, Thais Petrochelli ^{[2, 4]} ; Pereira, Gustavo Araujo ^{[1, 5]} ; Figueiredo, Mariana Cecchetto ^[2] ; Tasca Gois Ruiz, Ana Lucia ^{[2, 3]} ; Eberlin, Marcos Nogueira ^[6] ; de Carvalho, Joao Ernesto ^[7] ; Vendramini-Costa, Debora Barbosa ^[8] ; Pastore, Glaucia Maria ^[1]

Affiliation

^[1] Univ Estadual Campinas, Biofiavors & Bioact Cpds Lab, Dept Food Sci, Sch Food Engn, UNICAMP, Monteiro Lobato St 80, POB 13083-862, BR-13083862 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Chem Biol & Agr Pluridisciplinary Res Ctr CPQBA, UNICAMP, BR-13148218 Paulinia, SP - Brazil

^[3] Univ Estadual Campinas, UNICAMP, Grad Program Odontol, BR-13414903 Piracicaba, SP - Brazil

^[4] Univ Estadual Campinas, Inst Biol, UNICAMP, BR-13083862 Campinas, SP - Brazil

^[5] Fed Univ Para UFPA, Sch Food Engn FEA, Inst Technol ITEC, BR-66075110 Belem, Para - Brazil

^[6] Univ Estadual Campinas, Inst Chem, ThoMSon Mass Spectrometry Lab, UNICAMP, BR-13083970 Campinas, SP - Brazil

^[7] Univ Estadual Campinas, Fac Pharmaceut Sci, UNICAMP, BR-13083871 Campinas, SP - Brazil

^[8] Fox Chase Canc Ctr, 7701 Burholme Ave, Philadelphia, PA 19111 - USA

• Document type: Journal article
• Source: Food Research International; v. 133, JUL 2020.
• Web of Science Citations: 0

Abstract

Araticum (Annona crassiflora Mart.) is a native fruit from Brazilian Cerrado widely used by folk medicine. Nevertheless, the biological effects of its seeds and peel have not been extensively evaluated. We evaluate herein the antioxidant, antiproliferative and healing potential of araticum peel and seeds extracts. HPLC-ESI-MS/MS analysis showed flavonoids, namely epicatechin and quercetin, as the main compounds in peel and seeds extracts, respectively. These extracts showed high content of phenolic compounds (7254.46 and 97.74 mu g/g extract) and, as consequence, high antioxidant capacity. Interesting, the seeds extract was more effective than peel extract against all tested cancer cells, especially on NCI-ADR/RES (multidrug resistant ovary adenocarcinoma) cell line. In the cell migration assay by using HaCaT (keratinocyte), the seeds extract induced migration, while the peel extract showed an inhibitory effect. In this way, phenolic content could be related to antioxidant capacity, but it was not related to antiproliferative and healing effect. The araticum seeds extract showed an interesting response to in vitro biological assay although of its low content of phenolic compounds. Unidentified compounds, such as alkaloids and annonaceous acetogenins could be related to it. Araticum has potential to be used as therapeutic plant especially as antiproliferative and healing drug. (AU)

• FAPESP's process: 16/15746-6 - Evaluation of wound healing properties of araticum (Annona crassiflora Mart.)in vitro
• Grantee: Lívia Garcia Prado
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 18/10608-0 - Identification of bioactive compounds from Pitanga do Cerrado (Eugenia calycina Cambess) and study of its biological activities
• Grantee: Nayara Macedo Peixoto
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/50333-1 - COBRA: a novel compound discovery pipeline for rapid and cost-effective identification of bioactives with health beneficial effects in Brazilian crops
• Grantee: Glaucia Maria Pastore
• Support Opportunities: Research Projects - Thematic Grants