Oral prevalence and antifungal susceptibility of Candida species in cystic fibrosis patients

Author(s)

Souto Lepesqueur, Laura Soares ^[1] ; Tanaka, Marcia Hiromi ^[1] ; Gouvea Lima, Gabriela de Morais ^[1] ; Chiba, Sonia Mayumi ^[2] ; Mota, Adolfo Jose ^[3] ; Santos, Samara Ferreira ^[3] ; Koga-Ito, Cristiane Yumi ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Inst Sci & Technol, Av Engenheiro Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP - Brazil

^[2] Fed Univ Sao Paulo UNIFESP, Dept Pediat, Sao Paulo, SP - Brazil

^[3] Univ Fed Amazonas, Grad Program Biotechnol, Manaus, Amazonas - Brazil

• Document type: Journal article
• Source: ARCHIVES OF ORAL BIOLOGY; v. 116, AUG 2020.
• Web of Science Citations: 1

Abstract

Objective: This study aimed at assessing the oral prevalence ofCandida species in cystic fibrosis patients and the antifungal susceptibility of the isolates. Design: One hundred patients aged 3-20 years old were included in the study and were divided into three groups: G1 (low severity disease): 25 cystic fibrosis patients with Shwachman-Kulczycki score (SK) between 100 and 71; G2 (high severity disease): 25 cystic fibrosis patients with SK score under 40; and G3 (control): 50 healthy patients age- and gender-matched to cystic fibrosis patients. Stimulated saliva samples were collected and the oral fungal concentrations were assessed. Isolates were identified by phenotypic and genotypic tests. Antifungal susceptibilities to amphotericin B, flucytosine and fluconazole were determined by CLSI methodology. Fungal counts were compared by Kruskal Wallis and Dunn's test (5%). Results: A total of 68 % of Group 1, 80 % of Group 2, and 44 % of controls yielded positive Candida cultures. Oral concentrations of fungi were significantly higher in cystic fibrosis patients in relation to the control group (p < 0.0005). No significant difference was observed between low and high severity cystic fibrosis groups (p > 0.05). C. albicans was most frequently isolated species in all groups. Higher variability of Candida species was observed in the control group. C. dubliniensis and C. tropicalis were only detected among cystic fibrosis groups. All the isolates were susceptible to flucytosine and fluconazole. Conclusions: Patients with cystic fibrosis were more frequently colonized by Candida species and showed higher oral fungal burden. No antifungal resistant isolates were detected. (AU)

• FAPESP's process: 15/23318-1 - Effects of cystic fibrosis on the oral cavity of pediatric patients
• Grantee: Laura Soares Souto Lepesqueur
• Support Opportunities: Scholarships in Brazil - Doctorate