Transcriptome Profiling of Pacu (Piaractus mesopotamicus) Challenged With PathogenicAeromonas hydrophila: Inference on Immune Gene Response

Author(s)

Mastrochirico-Filho, Vito Antonio ^[1] ; Hata, Milene Elissa ^[1] ; Kuradomi, Rafael Yutaka ^[1] ; de Freitas, Milena Vieira ^[1] ; Ariede, Raquel Belini ^[1] ; Pinheiro, Daniel Guariz ^[2] ; Robledo, Diego ^[3] ; Houston, Ross ^[3] ; Hashimoto, Diogo Teruo ^[1]

Affiliation

^[1] Sao Paulo State Univ, UNESP, Aquaculture Ctr, Jaboticabal - Brazil

^[2] Sao Paulo State Univ, UNESP, Fac Agr & Vet Sci, Jaboticabal - Brazil

^[3] Univ Edinburgh, Royal Dick Sch Vet Studies, Edinburgh, Midlothian - Scotland

• Document type: Journal article
• Source: FRONTIERS IN GENETICS; v. 11, JUN 9 2020.
• Web of Science Citations: 0

Abstract

Pacu (Piaractus mesopotamicus) is a Neotropical fish of major importance for South American aquaculture. Septicemia caused byAeromonas hydrophilabacteria is currently considered a substantial threat for pacu aquaculture that have provoked infectious disease outbreaks with high economic losses. The understanding of molecular aspects on progress ofA. hydrophilainfection and pacu immune response is scarce, which have limited the development of genomic selection for resistance to this infection. The present study aimed to generate information on transcriptome of pacu in face ofA. hydrophilainfection, and compare the transcriptomic responses between two groups of time-series belonging to a disease resistance challenge, peak mortality (HM) and mortality plateau (PM) groups of individuals. Nine RNA sequencing (RNA-Seq) libraries were prepared from liver tissue of challenged individuals, generating similar to 160 million 150 bp pair-end reads. After quality trimming/cleanup, these reads were assembledde novogenerating 211,259 contigs. When the expression of genes from individuals of HM group were compared to individuals from control group, a total of 4,413 differentially expressed transcripts were found (2,000 upregulated and 2,413 downregulated candidate genes). Additionally, 433 transcripts were differentially expressed when individuals from MP group were compared with those in the control group (155 upregulated and 278 downregulated candidate genes). The resulting differentially expressed transcripts were clustered into the following functional categories: cytokines and signaling, epithelial protection, antigen processing and presentation, apoptosis, phagocytosis, complement system cascades and pattern recognition receptors. The proposed results revealing relevant differential gene expression on HM and PM groups which will contribute to a better understanding of the molecular defense mechanisms duringA. hydrophilainfection. (AU)

• FAPESP's process: 16/21011-9 - Integrated analysis of quantitative genetics and genomics to select genotypes of pacu (Piaractus mesopotamicus)
• Grantee: Diogo Teruo Hashimoto
• Support type: Regular Research Grants
• FAPESP's process: 17/26900-9 - RNA-seq and RAD-seq techniques for genetic characterization of pacu (Piaractus mesopotamicus) resistant to the bacteria Aeromonas hydrophila
• Grantee: Vito Antonio Mastrochirico Filho
• Support type: Scholarships abroad - Research Internship - Doctorate