Production of cellulases byAureobasidium pullulansLB83: optimization, characterization, and hydrolytic potential for the production of cellulosic sugars

Author(s)

Vieira, Matheus Maitan ^[1] ; Kadoguchi, Elen ^[1] ; Segato, Fernando ^[1] ; da Silva, Silvio S. ^[1] ; Chandel, Anuj K. ^[1]

Affiliation

^[1] Univ Sao Paulo, Engn Sch Lorena, Dept Biotechnol, Estr Municipal Campinho S-N, BR-12602810 Lorena, SP - Brazil

• Document type: Journal article
• Source: PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY; v. 51, n. 2 AUG 2020.
• Web of Science Citations: 0

Abstract

Aureobasidium pullulansLB83 was evaluated for cellulase production under submerged fermentation conditions. Different process variables such as carbon sources (corn cob, sugarcane bagasse, and sugarcane straw), synthetic (urea, ammonium sulfate, and peptone), and non-synthetic (soybean meal, rice, and corn meal) nitrogen sources and inoculum size were evaluated by one parameter at-a-time strategy.Aureobasidium pullulansLB83 showed maximum cellulase activity (FPase, 2.27 U/mL; CMCase, 7.42 U/mL) on sugarcane bagasse. Among the nitrogen sources, soybean meal as a non-synthetic nitrogen sources showed a maximum cellulase activity (FPase 2.45 U/mL; CMCase, 6.86 U/mL) after 60 hr. The inoculum size of 1.6 x 10(6) CFU/mL had the maximum FPase and CMCase activities of 3.14 and 8.74 U/mL, respectively. For the enzymatic hydrolysis, both the commercial cellulase (10 FPU/g of Cellic CTec 2 (\#A) and 10 FPU/g of crude enzyme extract (CEE) (\#B), and varying ratio of CTec 2 and CEE in combination \#C (5 FPU/g of CTec 2 + 5 FPU/g CEE), combination \#D (2.5 FPU/g of CTec 2 + 7.5 FPU/g CEE), and combination \#E (7.5 FPU/g of CTec 2 + 2.5 FPU/g CEE) were assessed for enzymatic hydrolysis of delignified sugarcane bagasse. Enzyme combination \#C showed maximum hydrolysis yield of 92.40%. The study shows the hydrolytic potential of cellulolytic enzymes fromA. pullulansLB83 for lignocellulosic sugars production from delignified sugarcane bagasse. (AU)

• FAPESP's process: 14/18714-2 - Enzymatic oxidation of sugarcane bagasse: discovery, characterization and new application of oxidative enzymes active in carbohydrates, applied to the enhancement of a fungal cell factory
• Grantee: Fernando Segato
• Support type: Program for Research on Bioenergy (BIOEN) - Young Investigators Grants
• FAPESP's process: 16/14852-7 - A sustainable solution against neglected tropical diseases using biosurfactants produced by yeasts in sugarcane bagasse hemicellulosic hydrolyzate
• Grantee: Paulo Ricardo Franco Marcelino
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
• Grantee: Roberto de Campos Giordano
• Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• FAPESP's process: 08/57926-4 - Research and development aiming at the integrated exploitation of sugarcane bagasse for the biotechnological production of lignocellulosic ethanol
• Grantee: Silvio Silvério da Silva
• Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants