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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Functional characterization of a novel thermophilic exo-arabinanase fromThermothielavioides terrestris

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Velasco, Josman [1] ; Oliva, Bianca [1] ; Goncalves, Aline Larissa [1] ; Lima, Awana Silva [1] ; Ferreira, Gislene [1] ; Franca, Bruno Alves [1] ; Mulinari, Evandro Jose [2] ; Goncalves, Thiago Augusto [3, 4] ; Squina, Fabio Marcio [4] ; Seiki Kadowaki, Marco Antonio [2] ; Maiorano, Alfredo [5] ; Polikarpov, Igor [2] ; de Oliveira, Leandro Cristante [6] ; Segato, Fernando [1]
Total Authors: 14
Affiliation:
[1] Univ Sao Paulo, Lorena Sch Engn, Dept Biotechnol, Lorena, SP - Brazil
[2] Univ Sao Paulo, Inst Fis Sao Carlos, Dept Fis & Ciencias Aplicadas, Sao Carlos, SP - Brazil
[3] Univ Estadual Campinas, Inst Biol, Dept Bioquim, Campinas, SP - Brazil
[4] Univ Sorocaba, Programa Proc Tecnol & Ambientais, Sorocaba, SP - Brazil
[5] Inst Pesquisas Tecnol Estado Sao Paulo SA, Nucleo Bionanomanufatura, Diretoria Operacoes & Negocios, Sao Paulo, SP - Brazil
[6] Sao Paulo State Univ UNESP, Dept Phys, Inst Biosci Humanities & Exact Sci, Sao Jose Do Rio Preto, SP - Brazil
Total Affiliations: 6
Document type: Journal article
Source: Applied Microbiology and Biotechnology; v. 104, n. 19 AUG 2020.
Web of Science Citations: 0
Abstract

Arabinanases from glycoside hydrolase family GH93 are enzymes with exo-activity that hydrolyze the alpha-1,5 bonds between arabinose residues present on arabinan. Currently, several initiatives aiming to use byproducts rich in arabinan such as pectin and sugar beet pulp as raw material to produce various compounds of interest are being developed. However, it is necessary to use robust enzymes that have an optimal performance under pH and temperature conditions used in the industrial processes. In this work, the first GH93 from the thermophilic fungusThermothielavioides terrestris(Abn93T) was heterologously expressed inAspergillus nidulans, purified and biochemically characterized. The enzyme is a thermophilic glycoprotein (optimum activity at 70 degrees C) with prolonged stability in acid pHs (4.0 to 6.5). The presence of glycosylation affected slightly the hydrolytic capacity of the enzyme, which was further increased by 34% in the presence of 1 mM CoCl2. Small-angle X-ray scattering results show that Abn93T is a globular-like-shaped protein with a slight bulge at one end. The hydrolytic mechanism of the enzyme was elucidated using capillary zone electrophoresis and molecular docking calculations. Abn93T has an ability to produce (in synergism with arabinofuranosidases) arabinose and arabinobiose from sugar beet arabinan, which can be explored as fermentable sugars and prebiotics. (AU)

FAPESP's process: 14/18714-2 - Enzymatic oxidation of sugarcane bagasse: discovery, characterization and new application of oxidative enzymes active in carbohydrates, applied to the enhancement of a fungal cell factory
Grantee:Fernando Segato
Support type: Program for Research on Bioenergy (BIOEN) - Young Investigators Grants
FAPESP's process: 17/16089-1 - Production of lignin chains, metagenomic screening and biocatalytic conversion of lignin to ferulic acid
Grantee:Thiago Augusto Gonçalves
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 14/06923-6 - Sugar cane biomass recalcitrance: basic knowledge related to the cell wall construction, pretreatment and enzymatic digestion, applied for the development of innovative biorefinery models
Grantee:Andre Luis Ferraz
Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants
FAPESP's process: 19/01165-0 - New recombinant PMOS from Aspergillus fumigatus: biochemical characterization and its effect on saccharification of sugarcane bagasse with different pre-treatments
Grantee:Josman Andrey Velasco Mendoza
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 15/50590-4 - Lignin valorization in cellulosic ethanol plants: biocatalytic conversion via ferulic acid to high value chemicals
Grantee:Fábio Márcio Squina
Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants
FAPESP's process: 19/06663-8 - Biochemical and functional studies of cellobiohydrolases from basidiomycetes of white-rot fungi with biotechnological potential
Grantee:Bianca Oliva
Support type: Scholarships in Brazil - Doctorate