Soymilk fermentation: effect of cooling protocol on cell viability during storage and in vitro gastrointestinal stress

Author(s)

Piazentin, Anna Carolina Meireles ^[1] ; da Silva, Thamires Maria Simoes ^[1] ; Florence-Franco, Ana Carolina ^[2] ; Bedani, Raquel ^[1] ; Converti, Attilio ^[3] ; de Souza Oliveira, Ricardo Pinheiro ^[1]

Affiliation

^[1] Univ Sao Paulo, Dept Biochem & Pharmaceut Technol, Prof Lineu Prestes, 580 Bloco 16, BR-05508000 Sao Paulo - Brazil

^[2] INSERM, Toulouse Purpan Pathophysiol Ctr, French Inst Hlth & Med Res, CPTP, Toulouse - France

^[3] Univ Genoa, Dept Civil Chem & Environm Engn, Pole Chem Engn, Via Opera Pia 15, I-16145 Genoa - Italy

• Document type: Journal article
• Source: Brazilian Journal of Microbiology; v. 51, n. 4 AUG 2020.
• Web of Science Citations: 0

Abstract

This work covers soymilk fermentation by starter and probiotic cultures and explores the influence of cooling protocol on cell viability, organic acid production, sugar consumption, fatty acid profile, and cell survival to in vitro gastrointestinal stress. After fermentation at 37 degrees C by mono- or co-cultures ofStreptococcus thermophilus(St),Lactobacillus bulgaricus(Lb), andLactobacillus paracasei(Lp), fermented soymilk was cooled directly at 4 degrees C for 28 days or cooled in two phases (TPC), i.e., by preceding that step by another at 25 degrees C for 8 h. Soybean milk fermentation by Lb alone lasted longer (15 h) than by StLb or StLbLp (9 h). In ternary culture, TPC increased Lp viability, linoleic, and lactic acid concentrations by 3.8, 22.6, and 96.2%, respectively, whereas the cooling protocol did not influence Lp and St counts after in vitro gastrointestinal stress. (AU)

• FAPESP's process: 19/19054-0 - Use of lactic acid bacteria for the production of probiotic dairy drinks and bacteriocins: application in the food and pharmaceutical industries
• Grantee: Ricardo Pinheiro de Souza Oliveira
• Support Opportunities: Research Grants - Visiting Researcher Grant - International