| Full text | |
| Author(s): Show less - |
Canto, Amanda M.
[1, 2]
;
Vieira, Andre S.
[2, 3]
;
Matos, Alexandre H. B.
[1, 2]
;
Carvalho, Benilton S.
[2, 4]
;
Henning, Barbara
[1, 2]
;
Norwood, Braxton A.
[5, 6]
;
Bauer, Sebastian
[6, 7]
;
Rosenow, Felix
[6, 7]
;
Gilioli, Rovilson
[8]
;
Cendes, Fernando
[2, 9]
;
Lopes-Cendes, Iscia
[1, 2]
Total Authors: 11
|
| Affiliation: | [1] Univ Estadual Campinas, Sch Med Sci, Dept Med Genet & Genom Med, UNICAMP, Campinas - Brazil
[2] Brazilian Inst Neurosci & Neurotechnol BRAINN, Campinas - Brazil
[3] Univ Estadual Campinas, Biol Inst, Dept Funct & Struct Biol, UNICAMP, Campinas - Brazil
[4] Univ Estadual Campinas, Dept Stat, Inst Math Stat & Sci Comp, UNICAMP, Campinas - Brazil
[5] Expesicor Inc, Kalispell, MT - USA
[6] Philipps Univ Marburg, Epilepsy Ctr Hessen, Dept Neurol, Marburg - Germany
[7] Goethe Univ Frankfurt, Epilepsy Ctr Frankfurt Rhine Main, Frankfurt - Germany
[8] Univ Estadual Campinas, Lab Anim Qual Control, UNICAMP, Campinas - Brazil
[9] Univ Estadual Campinas, Sch Med Sci, Dept Neurol, UNICAMP, Campinas - Brazil
Total Affiliations: 9
|
| Document type: | Journal article |
| Source: | SCIENTIFIC REPORTS; v. 10, n. 1 MAR 10 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Mesial temporal lobe epilepsy (MTLE) is a chronic neurological disorder affecting almost 40% of adult patients with epilepsy. Hippocampal sclerosis (HS) is a common histopathological abnormality found in patients with MTLE. HS is characterised by extensive neuronal loss in different hippocampus subregions. In this study, we used laser microdissection-based microproteomics to determine the protein abundances in different regions and layers of the hippocampus dentate gyrus (DG) in an electric stimulation rodent model which displays classical HS damage similar to that found in patients with MTLE. Our results indicate that there are differences in the proteomic profiles of different layers (granule cell and molecular), as well as different regions, of the DG (ventral and dorsal). We have identified new signalling pathways and proteins present in specific layers and regions of the DG, such as PARK7, RACK1, and connexin 31/gap junction. We also found two major signalling pathways that are common to all layers and regions: inflammation and energy metabolism. Finally, our results highlight the utility of high-throughput microproteomics and spatial-limited isolation of tissues in the study of complex disorders to fully appreciate the large biological heterogeneity present in different cell populations within the central nervous system. (AU) | |
| FAPESP's process: | 13/07559-3 - BRAINN - The Brazilian Institute of Neuroscience and Neurotechnology |
| Grantee: | Fernando Cendes |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |
| FAPESP's process: | 13/08635-5 - TRANSCRIPTOME ANALYSIS IN ANIMAL MODELS OF MESIAL TEMPORAL LOBE EPILEPSY |
| Grantee: | Alexandre Hilário Berenguer de Matos |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 15/12960-4 - Proteomic analysis of hippocampal tissues obtained from patients and animal model of Mesial Temporal Lobe Epilepsy: a comparative study |
| Grantee: | Amanda Morato Do Canto |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 16/19484-6 - Quantitative proteomic analysis of neuronal tissue obtained from patients and animal models of epilepsy |
| Grantee: | Barbara Henning Silva |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |