Gentamicin encapsulated within a biopolymer for the treatment ofStaphylococcus aureusandEscherichia coliinfected skin ulcers

Author(s)

Padua Gemeinder, Jose Lucio ^{[1, 2]} ; de Barros, Natan Roberto ^{[3, 2]} ; Pegorin, Giovana Sant'Ana ^{[3, 2]} ; Singulani, Junya de Lacorte ^[4] ; Borges, Felipe Azevedo ^{[3, 2]} ; Gabriel Del Arco, Marina Constante ^[5] ; Soares Mendes Giannini, Maria Jose ^[4] ; Fusco Almeida, Ana Marisa ^[4] ; de Souza Salvador, Sergio Luiz ^[5] ; Herculano, Rondinelli Donizetti ^[3]

Affiliation

^[1] Sao Paulo State Univ UNESP, Sch Sci Humanities & Languages, Dept Biotechnol, Assis, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Inst Chem, Dept Biochem & Chem Technol, Araraquara, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Biotechnol & Bioproc Engn, Araraquara, SP - Brazil

^[4] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Clin Anal, Araraquara, SP - Brazil

^[5] Sao Paulo Univ USP, Sch Pharmaceut Sci, Dept Clin Toxicol & Bromatol Anal, Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION; v. 32, n. 1 SEP 2020.
• Web of Science Citations: 0

Abstract

Skin wound infection requires carefully long-term treatment with an immense financial burden to healthcare systems worldwide. Various strategies such as drug delivery systems using polymer matrix from natural source have been used to enhance wound healing. Natural rubber latex (NRL) fromHevea brasiliensishas shown angiogenic and tissue repair properties. Gentamicin sulfate (GS) is a broad-spectrum antibiotic which inhibits the growth of a wide variety of microorganisms and, because of this, it has also been applied topically for treatment of local infections. The aim of this study was to develop a GS release system using NRL as matrix forStaphylococcus aureusandEscherichia coliinfected skin ulcers treatment, without changing drug antibiotic properties. The matrix did not change the GS antimicrobial activity againstS. aureusandE. colistrains. Moreover, the NRL-GS biomembrane did not exhibit hemolytic activity, being non-toxic to red blood cells. The eluates of NRL-GS biomembranes and GS solutions did not significantly reduce the survival ofCaenorhabditis elegansworms for 24 h at any of the tested concentrations. Thus, these results emphasize that the NRL-GS biomembrane proved to be a promising biomaterial for future studies on the development of dressings for topical uses, inexpensive and practicable, keeping drug antibiotic properties against pathogens and to reduce the side effects. (AU)

• FAPESP's process: 11/17411-8 - Evaluation of drugs delivery systems using natural rubber latex membranes as carrier
• Grantee: Rondinelli Donizetti Herculano
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/17526-8 - Development of a novel system employing natural rubber latex as releasing of vegetable extracts incorporated with nanoparticles: physical chemical and biological characterization
• Grantee: Rondinelli Donizetti Herculano
• Support Opportunities: Regular Research Grants