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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Transcutaneous Administration of Dengue Vaccines

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Author(s):
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Andreata-Santos, Robert [1] ; dos Santos Alves, Rubens Prince [1] ; Pereira, Sara Araujo [1] ; Pereira, Lennon Ramos [1] ; de Freitas, Carla Longo [1] ; Pereira, Samuel Santos [1] ; Venceslau-Carvalho, Alexia Adrianne [1] ; Castro-Amarante, Maria Fernanda [1] ; Pinho Favaro, Marianna Teixeira [1] ; Mathias-Santos, Camila [1] ; Amorim, Jaime Henrique [2] ; de Souza Ferreira, Luis Carlos [1]
Total Authors: 12
Affiliation:
[1] Univ Sao Paulo, Inst Biomed Sci, Microbiol Dept, Vaccine Dev Lab, BR-05508000 Sao Paulo - Brazil
[2] Fed Univ Western Bahia, Ctr Biol & Hlth Sci, BR-47810047 Barreiras, BA - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Viruses-Basel; v. 12, n. 5 MAY 2020.
Web of Science Citations: 0
Abstract

In the present study, we evaluated the immunological responses induced by dengue vaccines under experimental conditions after delivery via a transcutaneous (TC) route. Vaccines against type 2 Dengue virus particles (DENV2 New Guinea C (NGC) strain) combined with enterotoxigenic Escherichia coli (ETEC) heat-labile toxin (LT) were administered to BALB/c mice in a three-dose immunization regimen via the TC route. As a control for the parenteral administration route, other mouse groups were immunized with the same vaccine formulation via the intradermic (ID) route. Our results showed that mice vaccinated either via the TC or ID routes developed similar protective immunity, as measured after lethal challenges with the DENV2 NGC strain. Notably, the vaccine delivered through the TC route induced lower serum antibody (IgG) responses with regard to ID-immunized mice, particularly after the third dose. The protective immunity elicited in TC-immunized mice was attributed to different antigen-specific antibody properties, such as epitope specificity and IgG subclass responses, and cellular immune responses, as determined by cytokine secretion profiles. Altogether, the results of the present study demonstrate the immunogenicity and protective properties of a dengue vaccine delivered through the TC route and offer perspectives for future clinical applications. (AU)

FAPESP's process: 16/05570-8 - Use of Zika virus recombinant proteins for development of diagnostic methods and vaccines
Grantee:Lennon Ramos Pereira
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 12/50362-3 - Vaccines for dengue fever control delivered via intradermal and transcutaneous routes
Grantee:Luis Carlos de Souza Ferreira
Support type: Research Grants - Research Partnership for Technological Innovation - PITE
FAPESP's process: 16/20045-7 - Antigen discovery and development of serological diagnostic methods and vaccine approaches against the Zika Virus (ZIKV)
Grantee:Luis Carlos de Souza Ferreira
Support type: Research Projects - Thematic Grants
FAPESP's process: 14/17595-0 - New vaccine research based on recombinant proteins of dengue virus
Grantee:Luis Carlos de Souza Ferreira
Support type: Regular Research Grants
FAPESP's process: 16/23560-0 - Development of uniplex and multiplex diagnostic platforms for arbovirus infection using recombinant proteins
Grantee:Robert Andreata Santos
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 15/02352-7 - Vaccines for control of dengue: vaccine potential of the combination of the non-structural proteins in the generation of protective cell response in an experimental model
Grantee:Rubens Prince dos Santos Alves
Support type: Scholarships in Brazil - Doctorate