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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Multivariate probing of antitumor metal-based complexes damage on living cells through Raman imaging

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Author(s):
Mamian-Lopez, Monica Benicia [1, 2] ; Miguel, Rodrigo Bernardi [1] ; Araki, Koiti [1] ; Temperini, Marcia L. A. [1] ; da Costa Ferreira, Ana Maria [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Dept Quim Fundamental, Inst Quim, Av Prof Lineu Prestes 748, BR-05508000 Sao Paulo, SP - Brazil
[2] Fed Univ ABC, Av Estados 5001, BR-09210580 Santo Andre, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY; v. 244, JAN 5 2021.
Web of Science Citations: 0
Abstract

Intracellular modifications caused by two metal-based antitumor compounds were assessed by confocal Raman imaging assisted by multivariate curve resolution method, a very powerful deconvolution tool that can be used to extract the characteristic spectral profile of the individual or ``purest{''} components from an image dataset. The use of this Raman methodology has the advantage of being non-invasive and totally label-free. Four main different intracellular processes were observed under the Raman imaging and multivariate approach combination, and even, significant differences could be identified between the treatments with both metallodrugs. Leakage of the nucleus and nucleolus content into the cytoplasm, along with releasing of cytochrome c were observed for the treatment with the Cu-based complex. At the same time, changes of hydrogen-bonding network were also evidenced, indicating an apoptotic cellular death process, consistent with complementary Total Reflection X-Ray fluorescence (TXRF) and fluorescence experiments attesting mitochondria and DNA as main targets after uptake of the complex by cells. For treatment with the Zn-based complex, changes associated with cytochrome c were not detected, neither a rapid leakage of nucleus content upon 24 h treatment. The hydrogen-bonding network also followed a quite different pattern, suggesting that with this metallodrug, the cellular death follows a different mechanism. (C) 2020 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 14/07841-3 - Intensity and spectral fluctuations study in a single-molecule SERS spectra
Grantee:Monica Benicia Mamian Lopez
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 16/21070-5 - Vibrational spectroscopy with spatial resolution
Grantee:Mauro Carlos Costa Ribeiro
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 12/13119-3 - Vibrational spectroscopy in condensed phases
Grantee:Mauro Carlos Costa Ribeiro
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 18/21489-1 - Supramolecular nanotechnology: design, materials and devices
Grantee:Henrique Eisi Toma
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 13/07937-8 - Redoxome - Redox Processes in Biomedicine
Grantee:Ohara Augusto
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC