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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Physiological characterization of a new thermotolerant yeast strain isolated during Brazilian ethanol production, and its application in high-temperature fermentation

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Author(s):
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Prado, Cleiton D. [1] ; Mandrujano, Gustavo P. L. [1] ; Souza, Jonas. P. [1] ; Sgobbi, Flavia B. [1] ; Novaes, Hosana R. [1] ; da Silva, Joao P. M. O. [1] ; Alves, Mateus H. R. [1] ; Eliodorio, Kevy P. [2] ; Cunha, Gabriel C. G. [2] ; Giudici, Reinaldo [2] ; Procopio, Diele P. [2] ; Basso, Thiago O. [2] ; Malavazi, Iran [1] ; Cunha, Anderson F. [1]
Total Authors: 14
Affiliation:
[1] Univ Fed Sao Carlos UFSCar, Genet & Evolut Dept, BR-13565905 Sao Carlos, SP - Brazil
[2] Univ Sao Paulo, Escola Politecn, Chem Engn Dept, BR-05508010 Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: BIOTECHNOLOGY FOR BIOFUELS; v. 13, n. 1 OCT 27 2020.
Web of Science Citations: 0
Abstract

Background The use of thermotolerant yeast strains can improve the efficiency of ethanol fermentation, allowing fermentation to occur at temperatures higher than 40 degrees C. This characteristic could benefit traditional bio-ethanol production and allow simultaneous saccharification and fermentation (SSF) of starch or lignocellulosic biomass. Results We identified and characterized the physiology of a new thermotolerant strain (LBGA-01) able to ferment at 40 degrees C, which is more resistant to stressors as sucrose, furfural and ethanol than CAT-1 industrial strain. Furthermore, this strain showed similar CAT-1 resistance to acetic acid and lactic acid, and it was also able to change the pattern of genes involved in sucrose assimilation (SUC2 and AGT1). Genes related to the production of proteins involved in secondary products of fermentation were also differentially regulated at 40 degrees C, with reduced expression of genes involved in the formation of glycerol (GPD2), acetate (ALD6 and ALD4), and acetyl-coenzyme A synthetase 2 (ACS2). Fermentation tests using chemostats showed that LBGA-01 had an excellent performance in ethanol production in high temperature. Conclusion The thermotolerant LBGA-01 strain modulates the production of key genes, changing metabolic pathways during high-temperature fermentation, and increasing its resistance to high concentration of ethanol, sugar, lactic acid, acetic acid, and furfural. Results indicate that this strain can be used to improve first- and second-generation ethanol production in Brazil. (AU)

FAPESP's process: 16/10130-7 - Isolation, Molecular characterization and gene expression in termotholerant and ethanol resistant strains of Saccharomyces cerevisiae
Grantee:Anderson Ferreira da Cunha
Support type: Regular Research Grants
FAPESP's process: 18/17172-2 - How do fuel ethanol yeasts and contaminating lactic acid bacteria respond toward lignocellulosic-derived inhibitors?
Grantee:Thiago Olitta Basso
Support type: Regular Research Grants
FAPESP's process: 19/08393-8 - Experimental study and mathematical modeling of the alcoholic fermentation process considering thermal effects
Grantee:Kevy Pontes Eliodório
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 18/20697-0 - Selection and directed evolution of yeasts - Identification and analysis of important metabolic pathways for application in the bioethanol and fermented beverage industries.
Grantee:Anderson Ferreira da Cunha
Support type: Regular Research Grants