Epigenetic-mediated N-methyl-D-aspartate receptor changes in the brain of isolated reared rats

Author(s)

Loureiro, Camila Marcelino ^{[1, 2]} ; Fachim, Helene Aparecida ^{[3, 4]} ; Corsi-Zuelli, Fabiana ^[3] ; Shuhama, Rosana ^[3] ; Joca, Samia ^{[5, 6]} ; Menezes, Paulo Rossi ^[7] ; Dalton, Caroline F. ^[1] ; Del-Ben, Cristina Marta ^[3] ; Louzada-Junior, Paulo ^[2] ; Reynolds, Gavin P. ^[1]

Affiliation

^[1] Sheffield Hallam Univ, Biomol Sci Res Ctr, Sheffield, S Yorkshire - England

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Internal Med, Div Clin Immunol, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Neurosci & Behav, Sao Paulo - Brazil

^[4] Salford Royal Fdn Trust, Dept Endocrinol & Metab, Salford, Lancs - England

^[5] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept BioMol Sci, Sao Paulo - Brazil

^[6] Aarhus Univ, Aarhus Inst Adv Studies AIAS, Aarhus - Denmark

^[7] Univ Sao Paulo, Dept Prevent Med, Fac Med, Sao Paulo - Brazil

• Document type: Journal article
• Source: Epigenomics; v. 12, n. 22 NOV 2020.
• Web of Science Citations: 0

Abstract

Aim: We investigated: Grin1, Grin2a, Grin2b DNA methylation; NR1 and NR2 mRNA/protein in the prefrontal cortex (PFC); and hippocampus of male Wistar rats exposed to isolation rearing. Materials \& methods: Animals were kept isolated or grouped (n = 10/group) from weaning for 10 weeks. Tissues were dissected for RNA/DNA extraction and N-methyl-D-aspartate receptor subunits were analyzed using quantitative reverse transcription (RT)-PCR, ELISA and pyrosequencing. Results: Isolated-reared animals had: decreased mRNA in PFC for all markers, increased NR1 protein in hippocampus and hypermethylation of Grin1 in PFC and Grin2b in hippocampus, compared with grouped rats. Associations between mRNA/protein and DNA methylation were found for both brain areas. Conclusion: This study indicates that epigenetic DNA methylation may underlie N-methyl-D-aspartate receptor mRNA/protein expression alterations caused by isolation rearing. (AU)

• FAPESP's process: 13/08216-2 - CRID - Center for Research in Inflammatory Diseases
• Grantee: Fernando de Queiroz Cunha
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 16/12195-9 - Impact of early life stress in the inflammatory profile of first episode of psychosis (FEP) patients and in the animal model of post-weaning social isolation
• Grantee: Fabiana Maria das Graças Corsi Zuelli
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 12/05178-0 - Schizophrenia and other psychotic disorders: social and biological determinants
• Grantee: Paulo Rossi Menezes
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 13/11167-3 - Gene-environment interactions in Schizophrenia and other psychotic disorders
• Grantee: Rosana Shuhama
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 12/17626-7 - Cellular and molecular mechanisms involved in the role of atypical neurotransmitters in neuropsychiatric disorders
• Grantee: Francisco Silveira Guimaraes
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 15/02948-7 - Study of epigenetic alterations in GABAergic neurotransmission in schizophrenia and other psychoses and in animals undergoing social isolation from weaning
• Grantee: Helene Aparecida Fachim
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 19/13229-2 - Inflammatory profile in the general population: transdiagnostic dimensions in the context of the psychosis continuum
• Grantee: Fabiana Maria das Graças Corsi Zuelli
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/00624-5 - DNA methylation investigation in first episode of psychosis and in rats undergoing isolation rearing from weaning in the two major excitatory and inhibitory systems
• Grantee: Helene Aparecida Fachim
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor