High-throughput DNA sequencing of museum specimens sheds light on the long-missing species of the Bokermannohyla claresignata group (Anura: Hylidae: Cophomantini)

Author(s)

Lyra, Mariana L. ^{[1, 2]} ; Lourenco, Ana Carolina C. ^[3] ; Pinheiro, Paulo D. P. ^[4] ; Pezzuti, Tiago L. ^[5] ; Baeta, Delio ^{[1, 2, 6]} ; Barlow, Axel ^{[7, 8]} ; Hofreiter, Michael ^[7] ; Pombal Jr, Jose P. ; Haddad, Celio F. B. ^{[1, 2]} ; Faivovich, Julian ^{[9, 10]}

Affiliation

^[1] Univ Estadual Paulista, Dept Biodiversidade, UNESP, Av 24a 1515, BR-13506900 Rio Claro, SP - Brazil

^[2] Univ Estadual Paulista, Ctr Aquicultura, UNESP, IB, Av 24a 1515, BR-13506900 Rio Claro, SP - Brazil

^[3] Univ Estado Minas Gerais, Dept Ciencias Biol, Campus Uba, Ave Olegdrio Maciel 1427, BR-36502000 Uba, MG - Brazil

^[4] Univ Sao Paulo, Inst Biociencias, Dept Zool, Lab Anfibios, Rua Matao, Travessa 14, 101, Sala 137, Cidade Univ, BR-05508090 Sao Paulo, SP - Brazil

^[5] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Zool, Lab Herpetol, Av Presidente Antonio Carlos 6627, BR-31270901 Belo Horizonte, MG - Brazil

^[6] Univ Fed Rio de Janeiro, Dept Vertebrados, Museu Nacl, Setor Herpetol, BR-20940040 Rio De Janeiro, RJ - Brazil

^[7] Univ Potsdam, Inst Biochem & Biol, Dept Math & Nat Sci, Evolutionary Adapt Genom, Karl Liebknecht Str 24-25, D-14476 Potsdam - Germany

^[8] Nottingham Trent Univ, Sch Sci & Technol, Clifton Lane, Nottingham NG11 8NS - England

^[9] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Biodiversidad & Biol Expt, Buenos Aires, DF - Argentina

^[10] Consejo Nacl Invest Cient & Tecn, Museo Argentino Ciencias Nat Bernardino Rivadavia, Div Herpetol, Angel Gallardo 470, C1405DJR, Buenos Aires, DF - Argentina

• Document type: Journal article
• Source: ZOOLOGICAL JOURNAL OF THE LINNEAN SOCIETY; v. 190, n. 4, p. 1235-1255, DEC 2020.
• Web of Science Citations: 4

Abstract

The two species of the Bokermannohyla claresignata species group (Anura: Hylidae) have not been collected for the last four decades. It is the only species group of the hyline tribe Cophomantini that has not yet been analysed genetically. Its phylogenetic position is thus uncertain, and it has a combination of adult and larval character states that make this group a crucial missing piece that hinders our understanding of Cophomantini phylogenetics and character evolution. We obtained DNA sequences from a museum larval specimen of Bok. claresignata, using specialized extraction methods and high-throughput DNA sequencing, and combined the molecular phylogenetic results with available phenotypic information to provide new insights into the taxonomy and phylogenetic relationships of its species group. Our phylogenetic results place Bok. claresignata as sister to the Boana pulchella group, supporting its inclusion in Boana, together with Bokermannohyla clepsydra. In light of this new finding, we recognize a newly defined Boana claresignata group to accommodate these species, thus resolving both the polyphyly of Bokermannohyla and the paraphyly of Boana. Considering the phylogenetic relationships of the Boana claresignata group, we also discuss the evolution of suctorial tadpoles and mature oocyte/egg pigmentation in Cophomantini. (AU)

• FAPESP's process: 13/50741-7 - Diversity and conservation of Brazilian amphibians
• Grantee: Célio Fernando Baptista Haddad
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• FAPESP's process: 18/18473-6 - Study of comparative anatomy and evolution of the prepollical spine in Anura (Chordata: Lissamphibia)
• Grantee: Paulo Durães Pereira Pinheiro
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 12/25370-2 - Diversity and Evolution in Phyllomedusinae (Anura: Hylidae) and its relations with Pelodryadinae.
• Grantee: Délio Pontes Baêta da Costa
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/15425-0 - A multi-disciplinary approach to the study of amphibian diversification: phase 2
• Grantee: Taran Grant
• Support Opportunities: Research Grants - Young Investigators Grants - Phase 2