Does leukotriene F4 play a major role in the infection mechanism of Candida sp.?

Author(s)

Odir Rodrigues Melo, Carlos Fernando ^[1] ; Bachur, Luis Felipe ^[2] ; Delafiori, Jeany ^[1] ; Dabaja, Mohamed Ziad ^[1] ; de Oliveira, Diogo Noin ^[1] ; Guerreiro, Tatiane Melina ^[1] ; Tararam, Cibele Aparecida ^[3] ; Busso-Lopes, Ariane Fidelis ^[4] ; Moretti, Maria Luiza ^[5] ; Catharino, Rodrigo Ramos ^[1]

Affiliation

^[1] Univ Estadual Campinas, Sch Pharmaceut Sci, Innovare Biomarkers Lab, BR-13083877 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Hosp & Clin, Div Hosp Epidemiol, BR-13083888 Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Fac Med Sci, Mol Epidemiol & Infect Dis Lab, BR-13083888 Campinas, SP - Brazil

^[4] Brazilian Biosci Natl Lab, BR-13083970 Sao Paulo - Brazil

^[5] Univ Estadual Campinas, Fac Med Sci, Dept Internal Med, BR-13083888 Campinas, SP - Brazil

• Document type: Journal article
• Source: Microbial Pathogenesis; v. 149, DEC 2020.
• Web of Science Citations: 0

Abstract

Candidiasis is the most common fungal infection affecting hospitalized patients, especially immunocompromised and critical patients. Limitations regarding the assertive diagnosis of both Candidemia and Candidiasis not only impairs the introduction of effective treatments but also lays a heavy financial burden over the health system. Furthermore, it is still challenging to ascertain whether diagnostic methods are accurate and whether treatment is effective for patients with Candidemia. These constraints come from the uncertainty of the pathophysiological mechanism by which the pathogen establishes the opportunistic infection. Additionally, it is the reason why some patients present positive blood culture results, and others do not, and why it is very difficult during clinical routines to prove Candidemia or invasive candidiasis. Taking into account the current situation, this contribution proposes two markers that may help to understand the mechanisms of infection by the pathogen: Leukotriene F4 and 5,6-dihydroxy-eicosatetraenoic. These two lipids putatively modulate the host's immune response, and the initial data presented in this contribution suggest that these lipids allow the opportunistic infection to be installed. The study was carried out using an omics-based platform using direct-infusion high-resolution mass spectrometry and allied with bioinformatics tools to provide accurate and reliable results for biomarker candidates screening. (AU)

• FAPESP's process: 16/17066-2 - Metabolomics and lipidomics the Zika virus, from mosquitoes to the patient
• Grantee: Carlos Fernando Odir Rodrigues Melo
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 11/50400-0 - Mitochondrial energy metabolism, redox state and functionality in cell death and cardiometabolic and neurodegenerative disorders
• Grantee: Aníbal Eugênio Vercesi
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/03321-6 - A comprehensive metabolomics and lipidomics overview of plasma from patients with Alzheimer's Disease for screening dementia-linked biomarkers
• Grantee: Carlos Fernando Odir Rodrigues Melo
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 15/06809-1 - Determination of chemical markers in various bovine products using mass spectrometry
• Grantee: Rodrigo Ramos Catharino
• Support Opportunities: Regular Research Grants