| Full text | |
| Author(s): |
Wasinski, Frederick
[1]
;
Klein, Marianne O.
[1]
;
Bittencourt, Jackson C.
[1]
;
Metzger, Martin
[1]
;
Donato Jr, Jose
Total Authors: 5
|
| Affiliation: | [1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Fisiol & Biofis, Sao Paulo - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | Brain Research; v. 1751, JAN 15 2021. |
| Web of Science Citations: | 1 |
| Abstract | |
A growth hormone (GH) injection is able to induce the phosphorylated form of the signal transducer and activator of transcription 5 (pSTAT5) in a large number of cells throughout the mouse brain. The present study had the objective to map the distribution of GH-responsive cells in the brain of rats that received an intra-cerebroventricular injection of GH and compare it to the pattern found in mice. We observed that rats and mice exhibited a similar distribution of GH-induced pSTAT5 in the majority of areas of the telencephalon, hypothalamus and brainstem. However, rats exhibited a higher density of GH-responsive cells than mice in the horizontal limb of the diagonal band of Broca (HDB), supraoptic and suprachiasmatic nuclei, whereas mice displayed more GH-responsive cells than rats in the hippocampus, lateral hypothalamic area and dorsal motor nucleus of the vagus (DMX). Since both HDB and DMX contain acetylcholine-producing neurons, pSTAT5 was co-localized with choline acetyltransferase in GH-injected animals. We found that 50.0 +/- 4.5% of cholinergic neurons in the rat HDB coexpressed GH-induced pSTAT5, whereas very few co-localizations were observed in the mouse HDB. In contrast, rats displayed fewer cholinergic neurons responsive to GH in the DMX at the level of the area postrema. In summary, pSTAT5 can be used as a marker of GH-responsive cells in the rat brain. Although rats and mice exhibit a relatively similar distribution of GH-responsive neurons, some species-specific differences exist, as exemplified for the responsiveness to GH in distinct populations of cholinergic neurons. (AU) | |
| FAPESP's process: | 16/20897-3 - Role of orexin neurons as mediators of the central effects induced by growth hormone |
| Grantee: | Frederick Wasinski |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 16/02224-1 - The existence of neurogenesis during the lactation period |
| Grantee: | Jackson Cioni Bittencourt |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 17/16473-6 - Processing of aversive stimuli: circuitry between the laterodorsal tegmental nucleus, the habenula, and dorsal and median raphe nuclei |
| Grantee: | Martin Andreas Metzger |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 17/17998-5 - Evaluating chronic administration of melanin concentrating hormone (MCH) effects in lactating Long-Evans rats |
| Grantee: | Marianne Orlandini Klein |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 17/02983-2 - The role of growth hormone in the brain: relevance for neural functions and in disease |
| Grantee: | Jose Donato Junior |
| Support Opportunities: | Research Projects - Thematic Grants |