Spouted Bed Dried Rosmarinus officinalis Extract: A Novel Approach for Physicochemical Properties and Antioxidant Activity

Author(s)

Souza, Claudia R. F. ^[1] ; Baldim, Iara ^{[2, 1]} ; Bankole, Victor O. ^[1] ; da Ana, Raquel ^[3] ; Durazzo, Alessandra ^[4] ; Lucarini, Massimo ^[4] ; Cicero, Nicola ^{[5, 6]} ; Santini, Antonello ^[7] ; Souto, Eliana B. ^{[3, 2]} ; Oliveira, Wanderley P. ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Av Do Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Minho, CEB Ctr Biol Engn, Campus Gualtar, P-4710057 Braga - Portugal

^[3] Univ Coimbra, Fac Pharm, Dept Pharmaceut Technol, P-3000548 Coimbra - Portugal

^[4] CREA Res Ctr Food & Nutr, Via Ardeatina 546, I-00178 Rome - Italy

^[5] Univ Messina, Polo Univ Annunziata, Dept Biomed & Dent Sci & Morphofunct Imaging, I-98168 Messina - Italy

^[6] Science4Life, Vle Leonardo Sciascia Coop Fede Pal B, I-98168 Messina - Italy

^[7] Univ Napoli Federico II, Dept Pharm, Via Montesano 49, I-80131 Naples - Italy

• Document type: Journal article
• Source: AGRICULTURE-BASEL; v. 10, n. 8 AUG 2020.
• Web of Science Citations: 0

Abstract

In this study, a conical-cylindrical spouted bed dryer with Teflon(R)beads as spouting material was used for producing powdered rosemary (Rosmarinus officinalisL.) extract. The influence of the inlet drying gas temperature (T-gi) and the percentage ratio between the feed rate of concentrated liquid extract by the maximum evaporation capacity of the spouted bed (W-s/W-max) on selected physicochemical properties of the finished products were investigated. Antioxidant properties of the concentrated liquid extract and dried extracts were also evaluated by the 2.2-diphenyl-1-picrylhydrazyl radical scavenging (DPPH center dot) and lipid peroxidation induced by Fe2+/citrate (LPO) methods; and compared with the values obtained for a lyophilized extract (used as a control). Colloidal silicon dioxide (Tixosil(R)333) and maltodextrin (DE 14) at a 2:1 ratio was added to the concentrated extract before drying (4.4%w/w) to improve the drying performance. The drying variables W-s/W(max)and T(gi)have statistically significant influence on total polyphenols and total flavonoid contents of the dried powders. The concentrated extract (on dry basis-being absolute solid content) showed superior antioxidant activity (AA) compared to both the spouted bed dried and the lyophilized extracts; exhibiting IC50 values of 0.96 +/- 0.02, 2.16 +/- 0.04 and 3.79 +/- 0.05 mu g mL(-1)(DPPH(center dot)method) and 0.22 +/- 0.01, 1.31 +/- 0.01 and 2.54 +/- 0.02 mu g mL(-1)(LPO method), respectively. These results of AA are comparable to values obtained for quercetin, a flavonoid compound often used as a reference standard due to its potent antioxidant activity; with IC(50)of 1.17 mu g mL(-1)(DPPH center dot) and 0.22 mu g mL(-1)(LPO). However, the dried rosemary extracts are about 13.5 times more concentrated than the initial concentrated extract (dry weight), with a concentration of total flavonoids and polyphenols compounds ranging from 4.3 to 12.3 and from 1.2 to 4.7 times higher than the concentrated extract values (wet basis). The AA per dry product mass was thus significantly higher than the values measured for concentrated extractive solution, irrespective of some losses of AA apparently due to the drying process. (AU)

• FAPESP's process: 18/26069-0 - Encapsulation of essential oils for use in pharmaceutical compositions with antimicrobial activity
• Grantee: Wanderley Pereira Oliveira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/03427-2 - Microencapsulation and nanoencapsulation of natural compounds by drying technologies
• Grantee: Cláudia Regina Fernandes de Souza
• Support Opportunities: Scholarships in Brazil - Young Researchers
• FAPESP's process: 11/10333-1 - Microencapsulation and nanoencapsulation of natural compounds by drying technologies
• Grantee: Cláudia Regina Fernandes de Souza
• Support Opportunities: Research Grants - Young Investigators Grants