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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Stabilization of bilirubin oxidase in a biogel matrix for high-performance gas diffusion electrodes

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Sedenho, Graziela C. [1] ; Hassan, Ayaz [1] ; Macedo, Lucyano J. A. [1] ; Crespilho, Frank N. [1]
Total Authors: 4
[1] Univ Sao Paulo, Sao Carlos Inst Chem, Ave Trabalhador Sao Carlense 400, BR-13560970 Sao Carlos, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Journal of Power Sources; v. 482, JAN 15 2021.
Web of Science Citations: 1

Enzyme immobilization on solid conducting surfaces faces challenges for practical applications in technologies such as biosensors and biofuel cells. Short-term stability, poor electrochemical performance, and enzyme inhibition are some unsolved issues. Here, we show a simple methodology for bilirubin oxidase (BOD) immobilization on carbon-based gas diffusion electrode for four-electron electrochemical oxygen reduction reaction. BOD is incorporated into a Nafion (R) matrix and crosslinked with glutaraldehyde by a one-pot reaction in buffered solution, producing a stable BOD-based biogel. The biogel provides stable electrode performance and allows the direct electron-transfer mechanism of multicopper centers buried in the enzyme. A biocatalytic reduction current of -1.52 +/- 0.24 mA cm(-2) at 0.19 +/- 0.06 V was observed under gas diffusion conditions. Additionally, the bioelectrode showed unprecedented long-term stability under continuous operation combined with satisfactory catalytic current without redox mediator. The BOD-based biogel layer thickness and the entrapment of BOD into Nafion network are crucial for the biocathode stability, and BOD crosslinking by glutaraldehyde contributes to enhance the catalytic currents. Further, the BOD-based biogel provides a suitable microenvironment for long-term enzymatic activity involving three-phase interfacial reaction. The present study provides new insights into enzyme immobilization to overcome the critical short-term stability of enzyme-based electrochemical devices for practical applications. (AU)

FAPESP's process: 19/15333-1 - Bio-photo-electrochemical hybrid cells for solar energy conversion
Grantee:Frank Nelson Crespilho
Support type: Regular Research Grants
FAPESP's process: 19/12053-8 - High performance electrodes applied in organic batteries and in biofuel cell
Grantee:Frank Nelson Crespilho
Support type: Regular Research Grants
FAPESP's process: 15/22973-6 - Development of Microbial Fuel Biocells for Bioproduction and Oxidation of Ethanol
Grantee:Graziela Cristina Sedenho
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 17/20493-2 - Study of metalloenzymes through electrochemistry coupled to vibrational spectroscopy
Grantee:Lucyano Jefferson Alves de Macêdo
Support type: Scholarships in Brazil - Doctorate