Sensing of L-methionine in biological samples through fully 3D-printed electrodes

Author(s)

Kalinke, Cristiane ^[1] ; Neumsteir, Naile Vacionotto ^[1] ; de Oliveira, Paulo Roberto ^[2] ; Janegitz, Bruno Campos ^[2] ; Bonacin, Juliano Alves ^[1]

Affiliation

^[1] Univ Estadual Campinas, Inst Chem, BR-13083970 Campinas, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Nat Sci Math & Educ, BR-13600970 Araras, SP - Brazil

• Document type: Journal article
• Source: Analytica Chimica Acta; v. 1142, p. 135-142, JAN 15 2021.
• Web of Science Citations: 0

Abstract

The variation in biomarkers levels, such as L-methionine, can be an indicator of health problems or diseases, such as metabolism, neuropsychiatric disorders, or some virus infections. Thus, the development of accurate sensors, with low-cost and rapid response has been gaining increasing importance and attractiveness for the early diagnosis of diseases. In this regard, we have proposed a method for Lmethionine electrochemical detection using a low-cost and simple arrangement of 3D-printed electrodes (working, reference, and auxiliary electrodes) based on polylactic acid/graphene filament (PLA-G), in which all electrodes were printed. The working electrode was chemically and electrochemically treated, showing a high electroactive area, with graphene edge plans exposure and better electron transfer when compared to the untreated electrode. An excellent analytical performance was obtained with a sensitivity of 0.176 mAL mu mol(-1), a linear dynamic range of 5.0 mu mol L-1- 3000 mmol L-1 and limit of detection of 1.39 mmol L-1. The proposed device was successfully applied for L-methionine detection in spiked serum samples, showing satisfactory recovery values. This indicates the potentiality of the proposed arrangement of electrodes for the L-methionine detection in biological samples at different concentration levels. (C) 2020 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 17/23960-0 - Study of the mechanism of water oxidation by catalysts operating at pH7 and its incorporation in electrodes printed in 3D
• Grantee: Juliano Alves Bonacin
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/00473-2 - Development of 3-dimensional (3D) printed electrochemical biosensors with PLA polymer and graphene for the determination of biomolecules and diagnosis of diseases
• Grantee: Cristiane Kalinke
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/21097-3 - Bee-agriculture interactions: perspectives to sustainable use
• Grantee: Osmar Malaspina
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• FAPESP's process: 19/01844-4 - Development of nanostructured electrodes for determination of agrotoxic residues in honey
• Grantee: Paulo Roberto de Oliveira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 13/22127-2 - Development of novel materials strategic for integrated analytical devices
• Grantee: Lauro Tatsuo Kubota
• Support Opportunities: Research Projects - Thematic Grants