| Full text | |
| Author(s): |
Kalinke, Cristiane
[1]
;
Neumsteir, Naile Vacionotto
[1]
;
de Oliveira, Paulo Roberto
[2]
;
Janegitz, Bruno Campos
[2]
;
Bonacin, Juliano Alves
[1]
Total Authors: 5
|
| Affiliation: | [1] Univ Estadual Campinas, Inst Chem, BR-13083970 Campinas, SP - Brazil
[2] Univ Fed Sao Carlos, Dept Nat Sci Math & Educ, BR-13600970 Araras, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Analytica Chimica Acta; v. 1142, p. 135-142, JAN 15 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
The variation in biomarkers levels, such as L-methionine, can be an indicator of health problems or diseases, such as metabolism, neuropsychiatric disorders, or some virus infections. Thus, the development of accurate sensors, with low-cost and rapid response has been gaining increasing importance and attractiveness for the early diagnosis of diseases. In this regard, we have proposed a method for Lmethionine electrochemical detection using a low-cost and simple arrangement of 3D-printed electrodes (working, reference, and auxiliary electrodes) based on polylactic acid/graphene filament (PLA-G), in which all electrodes were printed. The working electrode was chemically and electrochemically treated, showing a high electroactive area, with graphene edge plans exposure and better electron transfer when compared to the untreated electrode. An excellent analytical performance was obtained with a sensitivity of 0.176 mAL mu mol(-1), a linear dynamic range of 5.0 mu mol L-1- 3000 mmol L-1 and limit of detection of 1.39 mmol L-1. The proposed device was successfully applied for L-methionine detection in spiked serum samples, showing satisfactory recovery values. This indicates the potentiality of the proposed arrangement of electrodes for the L-methionine detection in biological samples at different concentration levels. (C) 2020 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 17/23960-0 - Study of the mechanism of water oxidation by catalysts operating at pH7 and its incorporation in electrodes printed in 3D |
| Grantee: | Juliano Alves Bonacin |
| Support type: | Regular Research Grants |
| FAPESP's process: | 19/00473-2 - Development of 3-dimensional (3D) printed electrochemical biosensors with PLA polymer and graphene for the determination of biomolecules and diagnosis of diseases |
| Grantee: | Cristiane Kalinke |
| Support type: | Scholarships in Brazil - Post-Doctorate |
| FAPESP's process: | 17/21097-3 - Bee-agriculture interactions: perspectives to sustainable use |
| Grantee: | Osmar Malaspina |
| Support type: | Research Projects - Thematic Grants |
| FAPESP's process: | 19/01844-4 - Development of nanostructured electrodes for determination of agrotoxic residues in honey |
| Grantee: | Paulo Roberto de Oliveira |
| Support type: | Scholarships in Brazil - Post-Doctorate |
| FAPESP's process: | 13/22127-2 - Development of novel materials strategic for integrated analytical devices |
| Grantee: | Lauro Tatsuo Kubota |
| Support type: | Research Projects - Thematic Grants |