Hypoxia-cultured mouse mesenchymal stromal cells from bone marrow and compact bone display different phenotypic traits

Author(s):Show less -

Rossetti, Rafaela ^[1] ; Ros, Felipe Augusto ^[1] ; Botelho de Souza, Lucas Eduardo ^[1] ; Maconetto, Juliana de Matos ^[1] ; Mendes da Costa, Pericles Natan ^[1] ; Ferreira, Fernanda Ursoli ^[1] ; Borges, Josiane Serrano ^[1] ; de Carvalho, Julianne Vargas ^[1] ; Morotti, Nayara Patricia ^[1] ; Kashima, Simone ^[1] ; Covas, Dimas Tadeu ^[1]

Affiliation

^[1] Univ Sao Paulo, Blood Ctr Ribeirao Preto, Ribeirao Preto Med Sch, 2501 Tenente Catao Roxo Ave, BR-14051060 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: Experimental Cell Research; v. 399, n. 1 FEB 1 2021.
• Web of Science Citations: 0

Abstract

It has been suggested that the bone marrow microenvimnment harbors two distinct populations of mesenchymal stromal cells (MSC), one with a perivascular location and other present in the endosteum. A better understanding of the biology of these MSC subsets has been pursued in order to refine its clinical application. However, most comparative characterizations of mouse MSC have been performed in normoxia. This can result in misleading interpretations since mouse MSC subsets with low/defective p53 activity are known to be selected during culture in normoxia. Here, we report a comprehensive in vitro characterization of mouse MSC isolated from bone marrow (BM-MSC) and compact bone (CB-MSC) expanded and assayed under hypoxia for their morphology, clonogenic efficiency and differentiation capacity. We found that, under hypoxia, compact bone is richer in absolute numbers of MSC and isolation of MSC from compact bone is associated with a reduced risk of hematopoietic cell carryover. In addition, CB-MSC have higher in vitro osteogenic capacity than BM-MSC, while adipogenic differentiation potential is similar. These findings reinforce the hypothesis of the existence of MSC in bone marrow and compact bone representing functionally distinct cell populations and highlight the compact bone as an efficient source of murine MSC under physiological oxygen concentrations. (AU)

• FAPESP's process: 17/12663-5 - In vivo characterization of murine mesenchymal stem cells from bone marrow and compact bone in relation to its heterotopic hematopoietic niche generation potential
• Grantee: Rafaela Rossetti
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 15/19018-2 - In vitro characterization and in vivo analysis of the differentiation potential of murine mesenchymal stem cells from bone marrow and compact bone
• Grantee: Rafaela Rossetti
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 13/08135-2 - CTC - Center for Cell-Based Therapy
• Grantee: Dimas Tadeu Covas
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC