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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

High-Throughput Mega-Method for the Analysis of Pesticides, Veterinary Drugs, and Environmental Contaminants by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry and Robotic Mini-Solid-Phase Extraction Cleanup plus Low-Pressure Gas Chromatography-Tandem Mass Spectrometry, Part 2: Catfish

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Author(s):
Monteiro, Sergio H. [1, 2] ; Lehotay, Steven J. [2] ; Sapozhnikova, Yelena [2] ; Ninga, Ederina [2, 3] ; Lightfield, Alan R. [2]
Total Authors: 5
Affiliation:
[1] Environm Protect Res Ctr, Biol Inst, Pesticide Residue Lab, BR-04014900 Sao Paulo, SP - Brazil
[2] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 - USA
[3] Food Safety & Vet Inst, Dept Toxicol & Residues Monitoring, Tirana 1001 - Albania
Total Affiliations: 3
Document type: Journal article
Source: Journal of Agricultural and Food Chemistry; v. 69, n. 4, p. 1169-1174, FEB 3 2021.
Web of Science Citations: 8
Abstract

The goal of this study was to develop and validate a new method for simultaneous determination of 106 veterinary drugs and 227 pesticides and their metabolites plus 16 polychlorinated biphenyls (PCBs) at and below their regulatory levels established for catfish muscle in the European Union and U.S.A. To do this, two different QuEChERS-based methods for veterinary drugs and pesticides and PCBs were modified and merged into a single mega-method dubbed ``QuEChERSER{''} (more than QuEChERS), which is presented here for the first time. The mega-method was validated in catfish at four different spiking levels with 10 replicates per level. Sample extraction of 2 g test portions was made with 10 mL of 4:1 (v/v) acetonitrile/water, and then an aliquot was taken for ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) analysis of 106 veterinary drugs and 125 pesticides, including metabolites. The remaining extract after salting out was subjected to automated mini-solid-phase extraction cleanup (Instrument Top Sample Preparation) for immediate injection in low-pressure gas chromatography-tandem mass spectrometry (LPGC-MS/MS). The cleanup was conducted in parallel with the 10 min LPGC-MS/MS analysis for 167 PCBs, pesticides, and metabolites, which was conducted in parallel with the 10 min UHPLC-MS/MS analysis for 231 analytes to increase sample throughput (49 analytes were included in both techniques). In MS/MS, three ion transitions were monitored for nearly all targeted analytes to provide unambiguous identification as well as quantification. Satisfactory recoveries (70-120%) and relative standard deviations of <= 20% were achieved for 98 (92%) of the veterinary drugs and their metabolites and for 222 (91%) of pesticides, metabolites, and PCBs, demonstrating that the developed method is applicable for the analysis of these contaminants in fish as part of regulatory monitoring programs and other purposes. (AU)

FAPESP's process: 16/22370-2 - Automated high throughput sample preparation and analysis of multi-class contaminants in meat
Grantee:Sérgio Henrique Monteiro
Support Opportunities: Scholarships abroad - Research