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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Ethanol production process driving changes on industrial strains

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Nagamatsu, Sheila Tiemi [1, 2] ; Coutoune, Natalia [3] ; Jose, Juliana [2] ; Fiamenghi, Mateus Bernabe [2] ; Guimaraes Pereira, Goncalo Amarante [2] ; de Castro Oliveira, Juliana Velasco [3] ; Carazzolle, Marcelo Falsarella [2]
Total Authors: 7
[1] Yale Sch Med, Dept Psychiat, Div Human Genet, 333 Cedar St, New Haven, CT 06510 - USA
[2] Univ Estadual Campinas UNICAMP, Dept Genet Evolucao Microbiol & Imunol, Lab Genom & BioEnergia, Cidade Univ Zeferino Vaz, BR-13083970 Campinas, SP - Brazil
[3] Ctr Nacl Pesquisa Energia & Mat CNPEM, Lab Nacl Biorrenovaveis LNBR, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: FEMS Yeast Research; v. 21, n. 1 FEB 2021.
Web of Science Citations: 0

Ethanol production has key differences between the two largest producing countries of this biofuel, Brazil and the USA, such as feedstock source, sugar concentration and ethanol titers in industrial fermentation. Therefore, it is highly probable that these specificities have led to genome adaptation of the Saccharomyces cerevisiae strains employed in each process to tolerate different environments. In order to identify particular adaptations, in this work, we have compared the genomes of industrial yeast strains widely used to produce ethanol from sugarcane, corn and sweet sorghum, and also two laboratory strains as reference. The genes were predicted and then 4524 single-copy orthologous were selected to build the phylogenetic tree. We found that the geographic location and industrial process were shown as the main evolutionary drivers: for sugarcane fermentation, positive selection was identified for metal homeostasis and stress response genes, whereas genes involved in membrane modeling have been connected with corn fermentation. In addition, the corn specialized strain Ethanol Red showed an increased number of copies of MAL31, a gene encoding a maltose transporter. In summary, our work can help to guide new strain chassis selection for engineering strategies, to produce more robust strains for biofuel production and other industrial applications. (AU)

FAPESP's process: 17/02124-0 - Genetic engineering of Brazilian industrial strain Barra Grande for xylose fermentation
Grantee:Juliana Velasco de Castro Oliveira
Support type: Regular Research Grants
FAPESP's process: 13/08293-7 - CCES - Center for Computational Engineering and Sciences
Grantee:Munir Salomao Skaf
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 18/06254-8 - A stressful place for live: a deep understanding of selective pressures on yeast during alcoholic fermentations
Grantee:Mirta Natalia Coutouné
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 14/26905-2 - Bioinformatic analysis of QTLs mapping by high-throughput sequencing to identification of robustness mechanisms of industrial yeast
Grantee:Sheila Tiemi Nagamatsu
Support type: Scholarships in Brazil - Doctorate (Direct)