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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

An overview of open tubular liquid chromatography with a focus on the coupling with mass spectrometry for the analysis of small molecules

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Author(s):
Vargas Medina, Deyber Arley [1] ; Pereira dos Santos, Natalia Gabrielly [1] ; da Silva Burato, Juliana Soares [1] ; Basolli Borsatto, Joao Victor [1] ; Lancas, Fernando Mauro [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Inst Chem Sao Carlos, Sao Carlos, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Journal of Chromatography A; v. 1641, MAR 29 2021.
Web of Science Citations: 0
Abstract

Open tubular liquid chromatography (OT-LC) can provide superior chromatographic performance and more favorable mass spectrometry (MS) detection conditions. These features could provide enhanced sen-sitivity when coupled with electrospray ionization sources (ESI-) and lead to unprecedented detection ca-pabilities if interfaced with a highly structural informative electron ionization (EI) source. In the past, the exploitation of OT columns in liquid chromatography evolved slowly. However, the recent instrumental developments in capillary/nanoLC-MS created new opportunities in developing and applying OT-LC-MS. Currently, the analytical advantages of OT-LC-MS are mainly exploited in the fields of proteomics and biosciences analysis. Nevertheless, under the right conditions, OT-LC-MS can also offer superior chromatographic performance and enhanced sensitivity in analyzing small molecules. This review will provide an overview of the latest developments in OT-LC-MS, focusing on the wide variety of employed separation mechanisms, innovative stationary phases, emerging column fabrication technologies, and new OT formats. In the same way, the OT-LC \& rsquo;s opportunities and shortcomings coupled to both ESI and EI will be discussed, highlighting the complementary character of those two ionization modes to expand the LC \& rsquo;s detection boundaries in the performance of targeted and untargeted studies. (c) 2021 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 19/26263-4 - Development of open tubular capillary columns for micro and nano liquid chromatography coupled to sequential mass spectroscopy
Grantee:Natalia Gabrielly Pereira dos Santos
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 19/22724-7 - Novel instrumental approaches for automated and miniaturized analytical methods for the detection of organic compounds by liquid chromatography coupled to mass spectrometry
Grantee:Deyber Arley Vargas Medina
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 14/07347-9 - Development of novel materials and devices for miniaturized sample preparation techniques in the area of food safety
Grantee:Fernando Mauro Lanças
Support type: Regular Research Grants
FAPESP's process: 17/02147-0 - Single drop chromatography and its coupling to mass spectrometry: instrumental strategies, development of materials, automation and analytical applications
Grantee:Fernando Mauro Lanças
Support type: Research Projects - Thematic Grants
FAPESP's process: 14/03765-0 - Development of an automated solid-phase microextraction based on an arrangement with microsyringes packed sorbent for the simultaneous preparation of multiple samples
Grantee:Deyber Arley Vargas Medina
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 15/15462-5 - Employment of graphene as sorbent in miniaturized sample preparation techniques.
Grantee:Bruno Henrique Fumes
Support type: Scholarships in Brazil - Doctorate