Multifunctional hybrid nanoplatform based on Fe3O4@Ag NPs for nitric oxide delivery: development, characterization, therapeutic efficacy, and hemocompatibility

Author(s)

Pieretti, Joana Claudio ^[1] ; Goncalves, Marcelly Chue ^[2] ; Nakazato, Gerson ^[2] ; Santos de Souza, Ana Carolina ^[1] ; Boudier, Ariane ^[3] ; Seabra, Amedea Barozzi ^[1]

Affiliation

^[1] Fed Univ ABC UFABC, Ctr Nat & Human Sci CCNH, Santo Andre, SP - Brazil

^[2] Univ Estadual Londrina, Dept Microbiol, Londrina, PR - Brazil

^[3] Univ Lorraine, CITHEFOR, F-54000 Nancy - France

• Document type: Journal article
• Source: JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE; v. 32, n. 3 MAR 2021.
• Web of Science Citations: 0

Abstract

The combination of Fe3O4@Ag superparamagnetic hybrid nanoparticles and nitric oxide (NO) represents an innovative strategy for a localized NO delivery with a simultaneous antibacterial and antitumoral actions. Here, we report the design of Fe3O4@Ag hybrid nanoparticles, coated with a modified and nitrosated chitosan polymer, able to release NO in a biological medium. After their synthesis, physicochemical characterization confirmed the obtention of small NO-functionalized superparamagnetic Fe3O4@Ag NPs. Antibacterial assays demonstrated enhanced effects compared to control. Bacteriostatic effect against Gram-positive strains and bactericidal effect against E. coli were demonstrated. Moreover, NO-functionalized Fe3O4@Ag NPs demonstrated improved ability to reduce cancer cells viability and less cytotoxicity against non-tumoral cells compared to Fe3O4@Ag NPs. These effects were associated to the ability of these NPs act simultaneous as cytotoxic (necrosis inductors) and cytostatic compounds inducing S-phase cell cycle arrest. NPs also demonstrated low hemolysis ratio (<10%) at ideal work range, evidencing their potential for biomedical applications. {[}GRAPHICS] . (AU)

• FAPESP's process: 19/07766-5 - Blood compatibility of hybrid magnetic/silver nanoparticles coated with nitric oxide donor
• Grantee: Joana Claudio Pieretti
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• FAPESP's process: 18/08194-2 - Essential oil amended with metal nanoparticles functionalized with nitric oxide as a strategy to control plant pathogens in the agriculture
• Grantee: Amedea Barozzi Seabra
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/02832-7 - Hybrid magnetic and antibacterial nanoparticles: synthesis, characterization and applications
• Grantee: Joana Claudio Pieretti
• Support Opportunities: Scholarships in Brazil - Master