In Nasal Mucosal Secretions, Distinct IFN and IgA Responses Are Found in Severe and Mild SARS-CoV-2 Infection

Author(s):Show less -

Batista dos Santos, Juliana de Melo ^[1] ; Soares, Camila Pereira ^[2] ; Monteiro, Fernanda Rodrigues ^{[3, 4]} ; Mello, Ralyria ^[2] ; do Amaral, Jonatas Bussador ^[3] ; Aguiar, Andressa Simoes ^{[5, 6, 7]} ; Soledade, Mariana Pereira ^{[5, 6]} ; Sucupira, Carolina ^{[5, 6]} ; De Paulis, Milena ^[8] ; Andrade, Juliana Bannwart ^[8] ; Almeida, Flavia Jaqueline ^[9] ; Palazzi Safadi, Marco Aurelio ^[9] ; Mau, Luciana Becker ^{[10, 11]} ; Brasil, Jamile Menezes ^{[10, 11]} ; Ramalho, Theresa ^[12] ; Loures, V, Flavio ; Vieira, Rodolfo Paula ^{[13, 14, 1]} ; Durigon, Edison Luiz ^{[2, 15]} ; Leal de Oliveira, Danielle Bruna ^{[2, 15]} ; Lacerda Bachi, Andre Luis ^{[3, 16]}

Affiliation:Show less -

^[1] Univ Fed Sao Paulo, Postgrad Program Sci Human Movement & Rehabil, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Lab Clin & Mol Virol, Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Dept Otorhinolaryngol, Ear Nose & Throat ENT Lab, Sao Paulo - Brazil

^[4] Method Fac Sao Paulo, Sao Paulo - Brazil

^[5] Municipal Childrens Hosp Candido Fontoura, Infect Control Serv, Sao Paulo - Brazil

^[6] Municipal Childrens Hosp Candido Fontoura, Epidemiol Hosp Nucleo, Sao Paulo - Brazil

^[7] Sao Luiz Gonzaga Hosp St Casa Misericordia Sao Pa, Infect Control Serv, Sao Paulo - Brazil

^[8] Univ Sao Paulo, Univ Hosp, Sch Med, Dept Pediat, Sao Paulo - Brazil

^[9] St Casa Sao Paulo Sch Med Sci, Dept Pediat, Sao Paulo - Brazil

^[10] Menino Jesus Municipal Hosp, Infect Control Serv, Sao Paulo - Brazil

^[11] Menino Jesus Municipal Hosp, Epidemiol Hosp Nucleo, Sao Paulo - Brazil

^[12] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, Sao Paulo - Brazil

^[13] Brazilian Inst Teaching & Res Pulm & Exercise Imm, Sao Jose Dos Campos - Brazil

^[14] Univ Brasil, Postgrad Program Bioengn & Biomed Engn, Sao Paulo - Brazil

^[15] Sci Platform Pasteur Univ Sao Paulo, Sao Paulo - Brazil

^[16] Univ St Amaro, Postgrad Program Hlth Sci, Sao Paulo - Brazil

• Document type: Journal article
• Source: FRONTIERS IN IMMUNOLOGY; v. 12, FEB 25 2021.
• Web of Science Citations: 0

Abstract

Likely as in other viral respiratory diseases, SARS-CoV-2 elicit a local immune response, which includes production and releasing of both cytokines and secretory immunoglobulin (SIgA). Therefore, in this study, we investigated the levels of specific-SIgA for SARS-CoV-2 and cytokines in the airways mucosa 37 patients who were suspected of COVID-19. According to the RT-PCR results, the patients were separated into three groups: negative for COVID-19 and other viruses (NEGS, n = 5); negative for COVID-19 but positive for the presence of other viruses (OTHERS, n = 5); and the positive for COVID-19 (COVID-19, n = 27). Higher specific-SIgA for SARS-CoV-2, IFN-beta, and IFN-gamma were found in the COVID-19 group than in the other groups. Increased IL-12p70 levels were observed in OTHERS group as compared to COVID-19 group. When the COVID-19 group was sub stratified according to the illness severity, significant differences and correlations were found for the same parameters described above comparing severe COVID-19 to the mild COVID-19 group and other non-COVID-19 groups. For the first time, significant differences are shown in the airway's mucosa immune responses in different groups of patients with or without respiratory SARS-CoV-2 infection. (AU)

• FAPESP's process: 20/06409-1 - Evaluation of humoral immune response and inflammatory response in patients with confirmed diagnosis of COVID-19 at Hospital Sírio Libanês and correlation with disease severity
• Grantee: Edison Luiz Durigon
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/14115-0 - Influence of L-glutamine supplementation in the sarcopenia and immune/inflammatory response both systemic and in the respiratory tract of elderly practitioners or not of a regular program of combined physical exercises submitted to Influenza virus vaccine
• Grantee: André Luis Lacerda Bachi
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/20045-7 - Antigen discovery and development of serological diagnostic methods and vaccine approaches against the Zika Virus (ZIKV)
• Grantee: Luis Carlos de Souza Ferreira
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/23680-0 - Standardization of Sherlock technique (Specific High-Sensitivity Enzymatic Reporter Unlocking) using the Cas13a protein for the diagnosis of respiratory syncytial virus in pediatric patients
• Grantee: Camila Pereira Soares
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 12/15165-2 - Role of purinergic signaling and of SOCS-JAK-STAT signaling in the antiinflammatory effects of aerobic training in experimental models of asma and in asthmatic individuals
• Grantee: Rodolfo de Paula Vieira
• Support Opportunities: Research Grants - Young Investigators Grants