Multi-omics analysis provides insights into lignocellulosic biomass degradation by Laetiporus sulphureus ATCC 52600

Author(s):Show less -

de Figueiredo, Fernanda Lopes ^[1] ; de Oliveira, Ana Carolina Piva ^{[1, 2]} ; Terrasan, Cesar Rafael Fanchini ^[1] ; Goncalves, Thiago Augusto ^[1] ; Gerhardt, Jaqueline Aline ^[1] ; Tomazetto, Geizecler ^[3] ; Persinoti, Gabriela Felix ^[2] ; Rubio, Marcelo Ventura ^[1] ; Pena, Jennifer Andrea Tamayo ^[4] ; Araujo, Michelle Fernandes ^[4] ; de Carvalho Silvello, Maria Augusta ^[5] ; Franco, Telma Teixeira ^[6] ; Rabelo, Sarita Candida ^[7] ; Goldbeck, Rosana ^[5] ; Squina, Fabio Marcio ^[8] ; Damasio, Andre ^{[1, 9]}

Affiliation

^[1] Univ Campinas UNICAMP, Inst Biol, Dept Biochem & Tissue Biol, Campinas, SP - Brazil

^[2] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biorenewables Natl Lab LNBR, Campinas, SP - Brazil

^[3] Aarhus Univ, Dept Biol & Chem Engn BCE, DK-8200 Aarhus - Denmark

^[4] Univ Campinas UNICAMP, Chem Engn Sch, Campinas, SP - Brazil

^[5] Univ Campinas UNICAMP, Fac Food Engn, Dept Food Engn, Campinas, SP - Brazil

^[6] Univ Campinas UNICAMP, Interdisciplinary Ctr Energy Planning NIPE, Campinas, SP - Brazil

^[7] Sao Paulo State Univ UNESP, Dept Bioproc & Biotechnol, Coll Agr Sci, Botucatu, SP - Brazil

^[8] Univ Sorocaba UNISO, Dept Technol & Environm Proc, Sorocaba, SP - Brazil

^[9] Sao Paulo Fungal Grp, Sao Paulo - Brazil

• Document type: Journal article
• Source: BIOTECHNOLOGY FOR BIOFUELS; v. 14, n. 1 APR 17 2021.
• Web of Science Citations: 0

Abstract

Background Wood-decay basidiomycetes are effective for the degradation of highly lignified and recalcitrant plant substrates. The degradation of lignocellulosic materials by brown-rot strains is carried out by carbohydrate-active enzymes and non-enzymatic Fenton mechanism. Differences in the lignocellulose catabolism among closely related brown rots are not completely understood. Here, a multi-omics approach provided a global understanding of the strategies employed by L. sulphureus ATCC 52600 for lignocellulose degradation. Results The genome of Laetiporus sulphureus ATCC 52600 was sequenced and phylogenomic analysis supported monophyletic clades for the Order Polyporales and classification of this species within the family Laetiporaceae. Additionally, the plasticity of its metabolism was revealed in growth analysis on mono- and disaccharides, and polysaccharides such as cellulose, hemicelluloses, and polygalacturonic acid. The response of this fungus to the presence of lignocellulosic substrates was analyzed by transcriptomics and proteomics and evidenced the occurrence of an integrated oxidative-hydrolytic metabolism. The transcriptomic profile in response to a short cultivation period on sugarcane bagasse revealed 125 upregulated transcripts, which included CAZymes (redox enzymes and hemicellulases) as well as non-CAZy redox enzymes and genes related to the synthesis of low-molecular-weight compounds. The exoproteome produced in response to extended cultivation time on Avicel, and steam-exploded sugarcane bagasse, sugarcane straw, and Eucalyptus revealed 112 proteins. Contrasting with the mainly oxidative profile observed in the transcriptome, the secretomes showed a diverse hydrolytic repertoire including constitutive cellulases and hemicellulases, in addition to 19 upregulated CAZymes. The secretome induced for 7 days on sugarcane bagasse, representative of the late response, was applied in the saccharification of hydrothermally pretreated grass (sugarcane straw) and softwood (pine) by supplementing a commercial cocktail. Conclusion This study shows the singularity of L. sulphureus ATCC 52600 compared to other Polyporales brown rots, regarding the presence of cellobiohydrolase and peroxidase class II. The multi-omics analysis reinforces the oxidative-hydrolytic metabolism involved in lignocellulose deconstruction, providing insights into the overall mechanisms as well as specific proteins of each step. (AU)

• FAPESP's process: 19/21609-0 - Genetic engineering of Aspergillus niger for the production of a tailor-made enzymatic cocktail
• Grantee: Fernanda Lopes de Figueiredo
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/50612-8 - An integrated approach to explore a novel paradigm for biofuel production from lignocellulosic feedstocks
• Grantee: Telma Teixeira Franco
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/22669-0 - N-glycosylation and enzymes secretion in filamentous fungi
• Grantee: André Ricardo de Lima Damasio
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
• FAPESP's process: 20/05784-3 - EMU approved in grant 15 / 50590-4: chromatographic system and detectors for analysis of sugars and lignocellulosic monolignols
• Grantee: Fábio Márcio Squina
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 19/12860-0 - Deletion of N-glycosylation pathway genes in Aspergillus nidulans
• Grantee: Ana Carolina Piva de Oliveira
• Support Opportunities: Scholarships in Brazil - Technical Training Program - Technical Training
• FAPESP's process: 15/50590-4 - Lignin valorization in cellulosic ethanol plants: biocatalytic conversion via ferulic acid to high value chemicals
• Grantee: Fábio Márcio Squina
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants