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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cooperative and competitive antimicrobial photodynamic effects induced by a combination of methylene blue and curcumin

Full text
Author(s):
Dias, Lucas D. [1] ; Correa, Thaila Q. [1] ; Bagnato, Vanderlei S. [1, 2]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Sao Carlos Inst Phys, BR-13566590 Sao Carlos - Brazil
[2] Texas A&M Univ, Dept Biomed Engn, College Stn, TX 77843 - USA
Total Affiliations: 2
Document type: Journal article
Source: LASER PHYSICS LETTERS; v. 18, n. 7 JUL 2021.
Web of Science Citations: 0
Abstract

Alternatives techniques to combat bacteria and their resistance mechanisms are urgently needed in health and industrial sectors such as hospital environments and the food industry. Antimicrobial photodynamic therapy produces oxidative stress via multiple cell death mechanisms which are less prone to the development of resistance mechanisms by bacteria. We evaluate the simultaneous use of two photosensitizers (a nitrogen based-photosensitizer namely methylene blue and curcumin) at the same photodynamic protocol against Staphylococcus aureus bacteria and show their competitive and cooperative effects. We demonstrate that the use of two photosensitizers concomitantly used on bacterial reduction may produce effects that depend on: (a) attenuation of light by photosensitizers molecules; (b) shielding effect and steric hindrance by photosensitizers; (c) inter- and intra-photobleaching. Furthermore, we observe that the combined use of methylene blue and curcumin, both being photoactivated simultaneously (at 450 nm and 660 nm) is not an additive process. This study paves the ways and also shows the challenges for simultaneous use of two photosensitizers in an antimicrobial photodynamic protocol. (AU)

FAPESP's process: 14/50857-8 - National Institute in Basic Optics and Applied to Life Sciences
Grantee:Vanderlei Salvador Bagnato
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
Grantee:Vanderlei Salvador Bagnato
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 19/13569-8 - Study of the Mechanisms of Action in Photodynamic Therapy: From Photosensitizer to Practical Application
Grantee:Lucas Danilo Dias
Support Opportunities: Scholarships in Brazil - Post-Doctoral