Evaluation of immunohistopathological profile of tubular and solid canine mammary carcinomas

Author(s):Show less -

Borghes, Jessica ^[1] ; da Silva, Marcella Giancoli Kato Cano ^[1] ; Guimaraes, Katia de Oliveira Pimenta ^[1] ; Mario, Lara Carolina ^[1] ; da Anunciacao, Adriana Raquel de Almeida ^[1] ; Rabelo, Ana Carolina Silveira ^[1] ; Hayashi, Rafael Goncalves ^[1] ; Lima, Mariana Ferreira ^[1] ; Miglino, Maria Angelica ^[1] ; Favaron, Phelipe Oliveira ^[1] ; Carreira, Ana Claudia Oliveira ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo FMVZ USP, Sch Vet Med & Anim Sci, Dept Surg, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Med, NUCEL Cell & Mol Therapy Ctr, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Research in Veterinary Science; v. 136, p. 119-126, MAY 2021.
• Web of Science Citations: 0

Abstract

Breast cancer is the most common cancer in women, but the incidence of mammary carcinoma in female dogs is even higher than in humans. These two tumors have similarities that can be seen by its biological behavior, molecular genetic alterations, and histology. This suggest that female dogs can be an excellent model for preclinical oncological studies. And the mammary carcinoma most frequently found in this species is the tubular and solid carcinomas. The extracellular matrix (ECM) has an important role in the progression of these tumors. Because of that we proposed to evaluate the ECM components of these carcinomas through histology with specific stains such as Masson's Trichrome, Picrosirius Red and the technique of scanning electron microscopy. With that, we found the presence of collagen fibers in the tubular carcinoma and around its parenchyma. On the other hand, the solid carcinoma presented collagen fibers throughout the parenchyma and around each tumor cell. With the transmission electron microscopy, we observed the presence of mitochondrias and rough endoplasmic reticulum in both tumors. And finally, we evaluated the expression of proteins through the immunohistochemistry, in which we found a high expression of VEGF, PCNA, CK-18 and vimentin in solid carcinoma, and a positive mark in the tubular and solid carcinoma for collagen I, III and fibronectin. Thus, we demonstrated some differences in the ECM of these mammary carcinomas, allowing a better understanding of its histological characteristics, and these data may contribute to future studies about therapies focused on tumors ECM. (AU)

• FAPESP's process: 15/25815-2 - Canine mammary tumor characterization (tubular carcinoma) with emphasis on extracellular matrix
• Grantee: Marcella Giancoli Kato Cano da Silva
• Support Opportunities: Scholarships in Brazil - Scientific Initiation