| Full text | |
| Author(s): |
Serpeloni, Juliana Mara
[1]
;
Specian, Ana Flavia Leal
[1]
;
Ribeiro, Diego Luis
[1]
;
Tuttis, Katiuska
[1]
;
Heredia-Vieira, Silvia Cristina
[2]
;
Vilegas, Wagner
[3]
;
Martinez-Lopez, Wilner
[4]
;
Varanda, Eliana Aparecida
[5]
;
de Syllos Colus, Ilce Mara
[1]
Total Authors: 9
|
| Affiliation: | [1] State Univ Londrina UEL, Ctr Biol Sci, Dept Gen Biol, Londrina, Parana - Brazil
[2] Anhanguera Uniderp Univ, Nat Prod Lab, Campo Grande, MS - Brazil
[3] Sao Paulo State Univ UNESP, Expt Campus Sao Vicente, Sao Vicente - Brazil
[4] Inst Invest Biol Clemente Estable, Epigenet & Genom Instabil Lab, Montevideo - Uruguay
[5] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Biol Sci, Araraquara, SP - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | ENVIRONMENTAL TOXICOLOGY; v. 36, n. 8, p. 1544-1556, AUG 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Gastric cancer is the fifth most common malignancy worldwide. Serjania marginata Casar. (SM) displays anti-inflammatory properties and has been used to treat gastrointestinal disorders. In the current study, we examined whether the hydroethanolic extract of SM leaves exerted cytotoxic, mutagenic, and protective effects in non-tumor gastric epithelium cells (MNP01) and gastric adenocarcinoma cells (ACP02) in vitro and analyzed whether its action was selective. Initially, cell viability (MTT assay), cell cycle kinetics (flow cytometry), and cell proliferation (total protein content) were analyzed. In addition, genomic instability (cytokinesis-block micronucleus cytome assay), anti/pro-oxidant status (CM-H(2)DCFDA probe), and transcriptional expression (RT-qPCR) of genes related to cell cycle, cell death, and antioxidant defense were also evaluated. The SM extract was cytotoxic toward MNP01 and ACP02 cells at concentrations greater than 300 and 100 mu g center dot ml(-1), respectively, and decreased protein content only toward ACP02 cells at 200 mu g ml(-1). In ACP02 cells, the SM extract at 100 mu g center dot ml(-1) associated with doxorubicin (DXR; 0.2 mu g ml(-1)) clearly promoted cell cycle arrest at the G2/M phase. The extract alone was not mutagenic to either cell type and reversed DXR-induced DNA damage and H2O2-induced oxidative stress in MNP01 cells. The gene expression experiments showed that SM hydroethanolic extract exerts an antioxidant response via NFE2L2 activation in non-tumor gastric cells, and cell cycle arrest (G2/M) in ACP02 gastric cancer cells via the TP53 pathway. The selective action of SM indicates that it is a promising therapeutic agent to treat gastric diseases and merits further studies. (AU) | |
| FAPESP's process: | 12/01996-0 - Standardized phytoterapics for the treatment of chronic diseases: Cytotoxic, mutagenic and protective properties and the modulation of gene expression |
| Grantee: | Juliana Mara Serpeloni |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 09/52237-9 - Standardized herbal medicines for the treatment of chronic diseases |
| Grantee: | Wagner Vilegas |
| Support Opportunities: | BIOTA-FAPESP Program - Thematic Grants |