Isolation of diterpenes from Araucaria sp Brazilian brown propolis and development of a validated high-performance liquid chromatography method for its analysis

Author(s):Show less -

Santos, Mario Ferreira Conceicao ^[1] ; Oliveira, Larissa Costa ^[1] ; Ribeiro, Victor Pena ^[2] ; Soares, Marisi Gomes ^[3] ; Morae, Gabriel de Oliveira Isac ^[3] ; Sartori, Alan Giovanini de Oliveira ^[4] ; Rosalen, Pedro Luiz ^[5] ; Bastos, Jairo Kenupp ^[2] ; de Alencar, Severino Matias ^[4] ; Veneziani, Rodrigo Cassio Sola ^[1] ; Ambrosio, Sergio Ricardo ^[1]

Affiliation

^[1] Univ Franca, Nucleo Pesquisa Ciencias Exatas & Tecnol, Franca, SP - Brazil

^[2] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ribeirao Preto - Brazil

^[3] Fed Univ Alfenas UNIFAL MG, Chem Inst, Alfenas, MG - Brazil

^[4] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Agrifood Ind Food & Nutr, Piracicaba, SP - Brazil

^[5] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Piracicaba, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF SEPARATION SCIENCE; v. 44, n. 16 JUL 2021.
• Web of Science Citations: 1

Abstract

Propolis comprises a complex resinous product composed of plant's parts or exudates, pollen, bee wax, and enzymes. Brazilian brown propolis from Araucaria sp displays several biological activities. Considering the lack of validated analytical methods for its analysis, we are reporting the development of a validated high-performance liquid chromatography with photodiode array detector method to analyze Araucaria brown propolis. The crude propolis were extracted and chromatographed, furnishing six main diterpenes. The isolated standards were used to draw the analytical curves, allowing the studies of selectivity, precision, accuracy, recovery, robustness, the determination of limits of detection and limits of quantification. The mobile phase consisted of 0.1% acetic acid in water and acetonitrile, using an octadecylsilane column, 1 mL/min flow rate and detection at 200 or 241 nm. Relative standard deviation values obtained for intra-day and inter-day precision were lower than 4% for all diterpenes. From the five parameters for robustness, wavelength detection and flow rate were the critical ones. Limits of detection and quantification ranged from 0.808 to 10.359 mu g/mL and from 2.448 to 31.392 mu g/mL, respectively. The recoveries were between 105.03 and 108.13%, with relative standard deviation values around 5.0%. The developed method is precise, sensitive, and reliable for analyzing Araucaria brown propolis. (AU)

• FAPESP's process: 17/04138-8 - Attainment of chemical, analytical, biological, pharmacological and technological studies to fill the gaps on the development of Brazilian propolis sector
• Grantee: Jairo Kenupp Bastos
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/05080-6 - Multi-user equipment approved in grant 2017/04138-8 for high pressure semipreparative chromatographic system model LC-20AR
• Grantee: Sergio Ricardo Ambrosio
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 18/25010-2 - Isolation and identification of green and brown propolis constituents.
• Grantee: Mário Ferreira Conceição Santos
• Support Opportunities: Scholarships in Brazil - Post-Doctoral