Cytotoxic profile of CD3+CD20+T cells in progressive multiple sclerosis

Author(s)

Boldrini, Vinicius O. ^{[1, 2, 3]} ; Quintiliano, Raphael P. S. ^[3] ; Silva, Lucas S. ^[4] ; Damasceno, Alfredo ^[4] ; Santos, Leonilda M. B. ^{[1, 2, 3]} ; Farias, Alessandro S. ^{[1, 2, 3, 5]}

Affiliation

^[1] Natl Inst Sci & Technol Neuroimmunomodulat INCT N, Rio De Janeiro - Brazil

^[2] Univ Estadual Campinas, Inst Biol, Dept Genet Microbiol & Immunol, Autoimmune Res Lab, Campinas - Brazil

^[3] Univ Estadual Campinas, Inst Biol, Dept Genet Evolut Microbiol & Immunol, Neuroimmunol Unit, Campinas - Brazil

^[4] Univ Estadual Campinas, Dept Neurol, Campinas - Brazil

^[5] Expt Med Res Cluster, Div Immune Mediated Dis, Campinas - Brazil

• Document type: Letter
• Source: MULTIPLE SCLEROSIS AND RELATED DISORDERS; v. 52, JUL 2021.
• Web of Science Citations: 0

Abstract

Recently, it was shown that highly effective anti-CD20 therapies used for MS patients not only deplete CD20+ B cells, but also a small subset of T cells expressing CD20 surface marker (CD3+CD20+ T cells). Here we demonstrated that, in progressive MS patients, CD3+CD20+ T cells share the ability to express cytotoxic factors such as perforin and serine-protease granzyme-B (GzmB), classically associated with CD8+ T cells functionality. Beyond it, cluster analyses show that a set of activation markers and transcriptional factors related with CD8 effector program are also expressed in CD3+CD20+ T cells. Further characterization of surface and functional markers from CD3+CD20+ T subsets may be helpful for development of new therapeutic strategies mainly for progressive MS patients, as well as for assessing pathophysiological effects of highly effective anti-CD20 therapies. (AU)

• FAPESP's process: 17/21363-5 - Regulation of JAK/STAT/SOCS in the ontogen of IFNg-producing cells derivated from encephalitogenic Th17 cells during the clinical evolution of experimental autoimmune encephalomyelitis
• Grantee: Alessandro dos Santos Farias
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/06372-3 - Multiparametric flow cytometry
• Grantee: Alessandro dos Santos Farias
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 14/26431-0 - Multiple sclerosis, clinical study, neuropsychological, immunological, biomarkers and modifying-disease new drugs
• Grantee: Leonilda Maria Barbosa dos Santos
• Support Opportunities: Research Projects - Thematic Grants