Bacterial Photoinactivation Using PLGA Electrospun Scaffolds

Author(s):Show less -

Pereira, Aline O. ^[1] ; Lopes, Isabella M. I. ^[1] ; Silva, Thiago R. ^[1] ; Correa, Thaila Q. ^[2] ; Paschoalin, Rafaella T. ^{[3, 2]} ; Inada, Natalia M. ^[2] ; Iermak, Ievgeniia ^[2] ; Neto, Francisco van Riel ^[4] ; Araujo-Chaves, Juliana C. ^[5] ; Marletta, Alexandre ^[4] ; Tozoni, Jose R. ^[4] ; Mattoso, Luiz Henrique C. ^[3] ; Bagnato, Vanderlei S. ^[2] ; Nantes-Cardoso, Iseli L. ^[5] ; Oliveira Jr, Osvaldo N. ; Campana, Patricia T. ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Arts Sci & Humanities, BR-03828000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sao Carlos Inst Phys, BR-13560970 Sao Carlos - Brazil

^[3] Embrapa Instrumentat, Nanotechnol Natl Lab Agr LNNA, BR-13560970 Sao Carlos - Brazil

^[4] Fed Univ Uberlandia UFU, Inst Phys, BR-38408100 Uberlandia, MG - Brazil

^[5] Fed Univ ABC UFABC, Ctr Nat Sci & Humanities, BR-09210580 Santo Andre, SP - Brazil

• Document type: Journal article
• Source: ACS APPLIED MATERIALS & INTERFACES; v. 13, n. 27, p. 31406-31417, JUL 14 2021.
• Web of Science Citations: 0

Abstract

The use of ultraviolet (UV) and blue irradiation to sterilize surfaces is well established, but commercial applications would be enhanced if the light source is replaced with ambient light. In this paper, it is shown that nanofibers can be explored as an alternative methodology to UV and blue irradiation for bacterial inactivation. It is demonstrated that this is indeed possible using spun nanofibers of poly{[}lactic-co-(glycolic acid)] (PLGA). This work shows that PLGA spun scaffolds can promote photoinactivation of Staphylococcus aureus and Escherichia coli bacteria with ambient light or with laser irradiation at 630 nm. With the optimized scaffold composition of PLGA85:15 nanofibers, the minimum intensity required to kill the bacteria is much lower than in antimicrobial blue light applications. The enhanced effect introduced by PLGA scaffolds is due to their nanofiber structures since PLGA spun nanofibers were able to inactivate both S. aureus and E. coli bacteria, but cast films had no effect. These findings pave the way for an entirely different method to sterilize surfaces, which is less costly and environmentally friendly than current procedures. In addition, the scaffolds could also be used in cancer treatment with fewer side effects since photosensitizers are not required. (AU)

• FAPESP's process: 13/14262-7 - Nanostructured films from biologically-relevant materials
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 13/03609-6 - Polymeric scaffolds produced by electrospinning using poly-L-Lactic acid, PLLA
• Grantee: Thiago Reinaldos Silva
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 10/06728-8 - Introduction of growing factors into polymeric scaffolds for biomimetic tissues improvement.
• Grantee: Patricia Targon Campana
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/18725-2 - Hybrid core-shell and Janus nanofibers obtained by solution blow spinning
• Grantee: Rafaella Takehara Paschoalin
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/02317-2 - Interfaces in materials: electronic, magnetic, structural and transport properties
• Grantee: Adalberto Fazzio
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants