Antiglycation and antitumoral activity of Tribulus terrestris dry extract

Author(s)

Malaguti Figueiredo, Celia Cristina ^[1] ; Gomes, Amanda Costa ^[1] ; Granero, Filipe Oliveira ^[1] ; Bronzel Junior, Joao Luiz ^[1] ; Silva, Luciana Pereira ^[2] ; Tasca Gois Ruiz, Ana Lucia ^[3] ; Goncalves da Silva, Regildo Marcio ^{[1, 4]}

Affiliation

^[1] Sao Paulo State Univ UNESP, Inst Chem, Araraquara, SP - Brazil

^[2] Fundacao Educ Municlpio Assis FEMA, Assis, SP - Brazil

^[3] Univ Estadual Campinas, Fac Pharmaceut Sci, UNICAMP, Campinas, SP - Brazil

^[4] Sao Paulo State Univ UNESP, Sch Sci Humanities & Languages, Dept Biotechnol, Lab Herbal Med & Nat Prod, Assis, SP - Brazil

• Document type: Journal article
• Source: AVICENNA JOURNAL OF PHYTOMEDICINE; v. 11, n. 3, p. 224-237, MAY-JUN 2021.
• Web of Science Citations: 0

Abstract

Objective: Investigation of the antiglycation and antitumoral potential of standardized and saponins-enriched extracts of Tribulus terrestris herbal medicine. Materials and Methods: The procedures for the evaluation of the antiglycation activity of the standardized (TtSE) and saponins-enriched (TtEE) extracts of T. terrestris were: determination of relative mobility in electrophoresis (RME), free amino groups using OPA method and advanced glycation end-products (AGEs) fluorescence. Antioxidant activity was determined by DPPH radical scavenging test. In vitro antitumor activity of TtSE and TtEE was evaluated in human tumor cell lines. Results: The results were obtained by antiglycation tests (RME, OPA method and AGEs fluorescence determination), using BSA as protein and ribose as glycation agent, and antioxidant assay (DPPH test); it was verified that both extracts of T. terrestris have antiglycation and antioxidant activity. In addition, the extracts were able to induce death of more than 50% of human tumor cell lines. Conclusion: The present study showed that standardized and saponins-enriched extracts of T. terrestris herbal medicine present antiglycation and antioxidant and antiproliferative action in human tumor cells lines. The saponins-enriched extract proved a greater antiglycation and antioxidant activity in comparison to the standardized type. (AU)

• FAPESP's process: 15/17893-3 - Antioxidant and antiglicante activity of microencapsulation of the extract and saponins obtained from Tribulus terrestris
• Grantee: Célia Cristina Malaguti Figueiredo
• Support Opportunities: Scholarships in Brazil - Master