| Full text | |
| Author(s): |
Pinto, Leonardo
[1]
;
Soares, Guilherme
[1]
;
Prospero, Andre
[1]
;
Stoppa, Erick
[1]
;
Biasotti, Gabriel
[1]
;
Paixao, Fabiano
[2]
;
Santos, Armenio
[3]
;
Oliveira, Ricardo
[4]
;
Miranda, Jose
[1]
Total Authors: 9
|
| Affiliation: | [1] Sao Paulo State Univ, Biosci Inst Botucatu, Dept Biophys & Pharmacol, Prof Antonio Celso Wagner Zanin St, BR-18618689 Botucatu, SP - Brazil
[2] Univ Fed Sao Paulo, Sci & Technol Inst, Sao Jose Dos Campos - Brazil
[3] Univ Fed Ceara, Dept Physiol & Pharmacol, Fortaleza, Ceara - Brazil
[4] Univ Sao Paulo, Ribeirao Preto Med Sch, Ribeirao Preto - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | BIOMEDICAL ENGINEERING-BIOMEDIZINISCHE TECHNIK; v. 66, n. 4, p. 405-412, AUG 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
The identification of gastrointestinal (GI) motility disorders requires the evaluation of regional GI transit, and the development of alternative methodologies in animals has a significant impact on translational approaches. Therefore, the purpose of this study was to validate an easy and low-cost methodology (alternate current biosusceptometry - ACB) for the assessment of regional GI transit in rats through images. Rats were fed a test meal containing magnetic tracer and phenol red, and GI segments (stomach, proximal, medial and distal small intestine, and cecum) were collected to assess tracer's retention at distinct times after ingestion (0, 60, 120, 240, and 360 min). Images were obtained by scanning the segments, and phenol red concentration was determined by the sample's absorbance. The temporal retention profile, geometric center, gastric emptying, and cecum arrival were evaluated. The correlation coefficient between methods was 0.802, and the temporal retention of each segment was successfully assessed. GI parameters yielded comparable results between methods, and ACB images presented advantages as the possibility to visualize intrasegmental tracer distribution and the automated scan of the segments. The imaging approach provided a reliable assessment of several parameters simultaneously and may serve as an accurate and sensitive approach for regional GI research in rats. (AU) | |
| FAPESP's process: | 15/14923-9 - Instrumentation and applications by biosusceptometry alternating current: nanoparticles distribution studies, motility and quality control of solid dosage forms |
| Grantee: | José Ricardo de Arruda Miranda |
| Support Opportunities: | Regular Research Grants |