Characterization of multidrug-resistant and virulent Klebsiella pneumoniae strains belonging to the high-risk clonal group 258 (CG258) isolated from inpatients in northeastern Brazil

Author(s)

Nakamura-Silva, Rafael ^[1] ; Oliveira-Silva, Mariana ^[1] ; Rueda Furlan, Joao Pedro ^[2] ; Stehling, Eliana Guedes ^[2] ; Saraiva Miranda, Carlos Eduardo ^[3] ; Pitondo-Silva, Andre ^{[1, 3]}

Affiliation

^[1] Univ Ribeirao Preto, Postgrad Program Environm Technol, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[3] Univ Ribeirao Preto, UNAERP, Postgrad Program Dent, Bloco J, Lab 1, Av Costabile Romano 2201, BR-14096900 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: Archives of Microbiology; v. 203, n. 7, p. 4351-4359, SEP 2021.
• Web of Science Citations: 1

Abstract

Multidrug-resistant (MDR) and hypervirulent Klebsiella pneumoniae (hvKp) clones have become a major threat to global public health. The clonal group 258 (CG258) is considered a high-risk CG and the K. pneumoniae strains belonging to it are often multi-resistant and to spread mainly in the hospital environment. This study aimed to characterize the antimicrobial resistance profile, virulence factors, and the clonal relationships among 13 K. pneumoniae strains belonging to CG258 from patients admitted to a tertiary hospital in Teresina, in the state of Piaui, northeastern Brazil. Ten strains were classified as MDR and three as extensively drug-resistant (XDR). Three different beta-lactamase-encoding genes (bla(KPC), bla(OXA-1-like), and bla(CTX-M-Gp1)) and six virulence genes (fimH, ycfM, mrkD, entB, ybtS, and kfu) were detected. Moreover, two hypermucoviscous K. pneumoniae strains and one capsular K-type 2 were found. Multilocus sequence typing analysis revealed ten different sequence types (STs) (ST14, ST17, ST20, ST29, ST45, ST101, ST268, ST1800, ST3995, and ST3996) belonging to CG258, being two (ST3995 and ST3996) described for the first time in this study. (AU)

• FAPESP's process: 18/01890-3 - Study of resistance, virulence and epidemiological profile of Escherichia coli isolated from environment
• Grantee: João Pedro Rueda Furlan
• Support Opportunities: Scholarships in Brazil - Doctorate