Effect of Probiotics Lactobacillus acidophilus and Lacticaseibacillus rhamnosus on Antibacterial Response Gene Transcription of Human Peripheral Monocytes

Author(s)

Vale, Glauber Campos ^[1] ; Santana Mota, Brenda Izabela ^[1] ; Ando-Suguimoto, Ellen Sayuri ^[2] ; Alves Mayer, Marcia Pinto ^[2]

Affiliation

^[1] Univ Fed Piaui, Restorat Dent Dept, Teresina - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo - Brazil

• Document type: Journal article
• Source: PROBIOTICS AND ANTIMICROBIAL PROTEINS; AUG 2021.
• Web of Science Citations: 0

Abstract

Periodontitis and related systemic inflammatory diseases are characterized by imbalanced ratio between pro- and anti-inflammatory factors. Probiotics may control inflammation by altering the inflammatory phenotype of defense cells. We aimed to evaluate the gene transcription of the antibacterial response of monocytes to exposure to probiotic lactobacilli. CD14 + monocytes were obtained by positive selection from peripheral blood mononuclear cells from healthy donors (5 x 10(4) CD14 + /mL) and cultured with probiotic strains of Lacticaseibacillus rhamnosus (LR-32) and Lactobacillus acidophilus (LA-5) at a 1:10 multiplicity of infection in 24-well plates for 12 h. The gene expression analysis was performed by RT-qPCR using the Kit RT2 human antibacterial response, and in the supernatant, the cytokines were determined by ELISA. Tukey's post hoc test following an ANOVA with a p value of 5% was used for statistical analysis. Both probiotic strains increased the levels of cytokines TNF-alpha and CXCL-8 in the supernatant compared to the control of non-challenged cells (p < 0.05), but for IL-1 Beta and IL-6, this effect was observed only for LA-5 (p < 0.05). The fold-regulation values for the following genes for LA-5 and LR-32 were, respectively, IL-12B (431.94 and 33.30), IL-1 Beta (76.73 and 17.14), TNF-alpha (94.63 and 2.49), CXCL-8 (89.59 and 4.18), and TLR-2 (49.68 and 3.40). Likewise, most of the other genes evaluated showed greater expression for LA-5 compared to LR-32 (p < 0.05). The positive regulation of inflammatory factors such as IL-1 beta promoted by L. acidophilus LA-5 may increase the antibacterial activity of innate defense in periodontal tissues. However, this property may be deleterious by increasing inflammatory response. (AU)

• FAPESP's process: 15/18273-9 - New strategies for the control of periodontitis
• Grantee: Marcia Pinto Alves Mayer
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/10829-3 - Immunomodulatory effect of probiotic bacteria: analysis in peripheral monocytes and dendritic cells.
• Grantee: Gláuber Campos Vale
• Support Opportunities: Scholarships in Brazil - Post-Doctoral