Addition of EGCG to self-etching primer: effect on adhesive properties and bond stability to dentin

Author(s)

Fernandes, Fernando Pelegrim ^[1] ; Adorno, Carolina Cocco ^[1] ; da Silva, Tania Mara ^[2] ; Gomes Franca, Fabiana Mantovani ^[1] ; Turssi, Cecilia Pedroso ^[1] ; Basting, Rosanna Tarkany ^[1] ; Basting, Roberta Tarkany ^[1] ; de Paiva Goncalves, Sergio Eduardo ^[2] ; Botelho Amaral, Flavia Lucisano ^[1]

Affiliation

^[1] Inst Pesquisas Sao Leopoldo Mandic, Fac Sao Leopoldo Mandic, Jose Rocha Junqueira 13, BR-13045755 Campinas, SP - Brazil

^[2] Univ Estadual Paulista, UNESP, Inst Sci & Technol Sao Jose dos Campos, Sao Jose Dos Campos, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF ADHESION SCIENCE AND TECHNOLOGY; v. 35, n. 17, p. 1895-1908, SEP 2 2021.
• Web of Science Citations: 0

Abstract

This study evaluated how adding EGCG to a SEP (Clearfil SE Bond, Kuraray) affects the physicomechanical properties of adhesive, and the long-term microtensile bond strength (mu TBS) to dentin. Flexural strength (FS, n = 10) and degree of conversion (DC, n = 5) of the adhesive were analyzed after adding EGCG to SEP in two forms (powder or diluted in water), and in different concentrations (0.00%-control, 0.01%, 0.02% and 0.05%). The mu TBS was tested by randomly dividing dentin fragments into 4 groups (n = 10): EGCG-SEP: EGCG (0.01%) added to SEP; EGCG-PRE: EGCG (0.01%) dentin pretreatment; CHX-PRE: 2% chlorhexidine dentin pretreatment; SEP: SEP applied without modification. After restoration of the dentin surface, beam-shaped specimens were submitted to mu TBS after 24 h or 12 months of storage in artificial saliva. Particle size (PS), polydispersity index (PDI) and zeta potential (ZP) of EGCG-SEP and SEP solutions were measured at different storage periods (baseline, 14 or 28 days). Data was submitted to ANOVA or Kruskal-Wallis tests (alpha = 0.05). FS (p = 0.485) and DC (p = 0.6067) were not different among the groups. CHX-PRE and EGCG-PRE had significantly higher mu TBS means than EGCG-SEP, but none differed from SEP (p = 0.053). mu TBS decreased significantly after 12 months of storage (p < 0.001). PS (p < 0.001), and ZP (p = 0.003) means in EGCG-SEP were statistically higher and more negative, respectively, than SEP. PDI did not differ among the groups (p > 0.05). Incorporation of EGCG into SEP did not change adhesive properties; however, it influenced solution stability and did not prevent the decrease in long-term mu TBS to dentin. (AU)

• FAPESP's process: 16/04135-6 - Effect of distinct incorporation mechanism and epigallocatequin gallate concentrations on two-step self-etching adhesive's flexural resistance and degree of conversion
• Grantee: Carolina Côcco Adorno
• Support Opportunities: Scholarships in Brazil - Scientific Initiation