A Missense Mutation in the MYBPH Gene Is Associated With Abdominal Fat Traits in Meat-Type Chickens

Author(s)

Trevisoli, Priscila Anchieta ^[1] ; Monteiro Moreira, Gabriel Costa ^[1] ; Boschiero, Clarissa ^[1] ; Mello Cesar, Aline Silva ^[2] ; Petrini, Juliana ^[1] ; Alves Margarido, Gabriel Rodrigues ^[3] ; Ledur, Monica Correa ^[4] ; Mourao, Gerson Barreto ^[1] ; Garrick, Dorian ^[5] ; Coutinho, Luiz Lehmann ^[1]

Affiliation

^[1] Univ Sao Paulo, Anim Sci Dept, Luiz de Queiroz Coll Agr ESALQ, Piracicaba - Brazil

^[2] Univ Sao Paulo, Agrifood Ind Food & Nutr Dept, Luiz de Queiroz Coll Agr ESALQ, Piracicaba - Brazil

^[3] Univ Sao Paulo, Dept Genet, Luiz de Queiroz Coll Agr ESALQ, Piracicaba - Brazil

^[4] Embrapa Suinos & Aves, Concordia - Brazil

^[5] Massey Univ, Sch Agr, Wellington - New Zealand

• Document type: Journal article
• Source: FRONTIERS IN GENETICS; v. 12, AUG 11 2021.
• Web of Science Citations: 1

Abstract

Chicken is an important source of protein for human nutrition and a model system for growth and developmental biology. Although the genetic architecture of quantitative traits in meat-type chickens has been the subject of ongoing investigation, the identification of mutations associated with carcass traits of economic interest remains challenging. Therefore, our aim was to identify predicted deleterious mutation, which potentially affects protein function, and test if they were associated with carcass traits in chickens. For that, we performed a genome-wide association analysis (GWAS) for breast, thigh and drumstick traits in meat-type chickens and detected 19 unique quantitative trait loci (QTL). We then used: (1) the identified windows; (2) QTL for abdominal fat detected in a previous study with the same population and (3) previously obtained whole genome sequence data, to identify 18 predicted deleterious single nucleotide polymorphisms (SNPs) in those QTL for further association with breast, thigh, drumstick and abdominal fat traits. Using the additive model, a predicted deleterious SNP c.482C > T (SIFT score of 0.4) was associated (p-value < 0.05) with abdominal fat weight and percentage. This SNP is in the second exon of the MYBPH gene, and its allele frequency deviates from Hardy-Weinberg equilibrium. In conclusion, our study provides evidence that the c.482C > T SNP in the MYBPH gene is a putative causal mutation for fat deposition in meat-type chickens. (AU)

• FAPESP's process: 14/21380-9 - Genome wide association studies for fat deposition and carcass yield in chickens
• Grantee: Gabriel Costa Monteiro Moreira
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/13589-0 - Validation and association of non tolerated SNPs with muscle growth and fat deposition traits in chickens
• Grantee: Priscila Anchieta Trevisoli
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 16/00569-1 - IMPUTATION AND GENOME-WIDE ASSOCIATION ANALYSIS USING HAPLOTYPES FOR ABDOMINAL FAT IN CHICKENS.
• Grantee: Gabriel Costa Monteiro Moreira
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate