Multidrug resistance IncC plasmid carrying bla(CMY-97) in Shiga toxin-producing Escherichia coli ST215-H54 of ovine origin

Author(s)

Furlan, Joao Pedro Rueda ^[1] ; Lopes, Ralf ^[1] ; Stehling, Eliana Guedes ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, Sao Paulo - Brazil

• Document type: Journal article
• Source: INFECTION GENETICS AND EVOLUTION; v. 93, SEP 2021.
• Web of Science Citations: 0

Abstract

CMY-type beta-lactamases are the most reported plasmid-mediated AmpC (pAmpC), with the CMY-2-like group being the most clinically relevant described in Escherichia coli at human-animal-environment interface. Shiga toxin-producing E. coli (STEC) lineages are zoonotic pathogens commonly reported causing serious clinical conditions in humans, including severe diarrheagenic diseases. Therefore, this study aimed to investigate a multidrug-resistant (MDR) STEC isolate (A313) recovered from a healthy sheep and carrying mobile bla(CMY-97), that encodes a pAmpC belonging to the CMY-2-like group. The A313 isolate exhibited a MDR profile to clinically relevant antimicrobials (i.e., cephalosporins, aminoglycosides, and fluomquinolones), but reduced susceptibility to extended-spectrum cephalosporins and aztreonam. Besides, virulence genes (stx2, gad and iutA) were detected in A313, which belonged to ST215/CC10 and phylogenetic group A, whereas the fimH54 was identified. The bla(CMY-97) gene and other antimicrobial resistance determinants {[}cuph(6)-Id, cuph(3 `')-Ib, aac(3)-IId, aadA5, floR, tetA, sul1, and su12], as well as genes encoding tolerance to mercury (merRTPCADE), were harbored by an IncC plasmid (named pA313-CMY-97, similar to 176 kb). A novel genetic context of bla(CMY-2-like), in which a 208-bp ISEcp1 was truncated by an IS26 in the opposite orientation upstream of the bla(CMY-97) gene (1526- Delta ISEcp1-bla(CMY-97)-blc-sugE-encR), was also identified in pA313-CMY-97. To the best of our knowledge, this is the first report on the acquisition of bla(CMY-97) into a plasmid. Therefore, we reported ovine as reservoir of clinically relevant MDR bacteria carrying mobile bla(CMY-97) with potential for zoonotic transmission. (AU)

• FAPESP's process: 18/19539-0 - Molecular characterization of Escherichia coli isolates from the environment
• Grantee: Eliana Guedes Stehling
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/01890-3 - Study of resistance, virulence and epidemiological profile of Escherichia coli isolated from environment
• Grantee: João Pedro Rueda Furlan
• Support Opportunities: Scholarships in Brazil - Doctorate