Saccharification of different sugarcane bagasse varieties by enzymatic cocktails produced by Mycothermus thermophilus and Trichoderma reesei RP698 cultures in agro-industrial residues

Author(s)

Scarcella, Ana Silvia de Almeida ^[1] ; Pasin, Thiago Machado ^[1] ; de Oliveira, Tassio Brito ^[2] ; de Lucas, Rosymar Coutinho ^{[1, 2]} ; Ferreira-Nozawa, Monica Stropa ^[2] ; de Freitas, Emanuelle Neiverth ^[1] ; Vici, Ana Claudia ^[2] ; Buckeridge, Marcos Silveira ^[3] ; Michelin, Michele ^[4] ; Polizeli, Maria de Lourdes Teixeira de Moraes ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Bioquim & Imunol, Bandeirantes Ave 3900, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto FF, Dept Biol, Bandeirantes Ave 3900, BR-14040901 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Inst Biociencias, Dept Bot, Matao St 277, BR-05508090 Sao Paulo - Brazil

^[4] Univ Minho, Ctr Engn Biol, Campus Gualtar, P-4710057 Braga - Portugal

• Document type: Journal article
• Source: ENERGY; v. 226, JUL 1 2021.
• Web of Science Citations: 0

Abstract

Lignocellulosic biomasses are promising sources for the obtaining of clean energy through their bioconversion into ethanol. Their sacchariflcation involves a multi-enzymatic system. Here, we evaluated the hydrolysis of a mixture of sugarcane bagasse varieties (SCB), Energy cane fiber (EC), and sugarcane bagasse from the SP80-3280 variety, all in natura, using fungal enzymatic extracts obtained from Mycothermus thermophilus and Trichoderma reesei RP698 cultures supplemented with various agroindustrial residues. The enzymatic extracts from both fungi, when grown in a corn cob and corn straw, led to the highest sugarcane hydrolysis. For M. thermophilus, the reducing sugars released (mg/mL) were 1.21 +/- 0.12, 1.25 +/- 0.14, and 0.98 +/- 0.05 for SCB, EC and SP80-3280, respectively; for T. reesei, the reducing sugars were 0.84 +/- 0.08, 0.89 +/- 0.06 and 0.68 +/- 0.03 for SCB, SP80-3280, and EC, respectively. The cocktail obtained from the co-cultivation of these fungi in corn straw at 35 degrees C showed the best hydrolysis results, the release of sugars (mg/mL) was 1.31 +/- 0.06 (SCB), 2.18 +/- 0.08 (EC) and 1.67 +/- 0.09 (SP80-3280). Scanning electron microscopy and thermogravimetric analysis confirmed changes in the fiber structures after enzymatic hydrolysis. Thus, these fungi were shown to be promising for an enzymatic cocktail design and sugarcane biomass sacchariflcation. (C) 2021 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 14/50884-5 - INCT 2014: National Institute of Science and Technology of Bioethanol
• Grantee: Marcos Silveira Buckeridge
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/07522-6 - Development of eco-friendly technologies for the hydrolysis of biomass and residues of cellulose pulp and paper industry
• Grantee: Maria de Lourdes Teixeira de Moraes Polizeli
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/09000-4 - Effects of temperature and water stress, in climate change scenario, on the fungal community in tropical soil and selection of nitrous oxide producing strains
• Grantee: Tássio Brito de Oliveira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral