| Full text | |
| Author(s): Show less - |
Romero, Stefhani Andrioli
[1]
;
Betim Pavan, Isadora Carolina
[2]
;
Morelli, Ana Paula
[1]
;
Silva Mancini, Mariana Camargo
[1]
;
Salvino da Silva, Luiz Guilherme
[1]
;
Fagundes, Isabella
[1]
;
Rocha Silva, Cayo Henrique
[1]
;
Saboia Ponte, Luis Gustavo
[1]
;
Rostagno, Mauricio Ariel
[1]
;
Neves Bezerra, Rosangela Maria
[1]
;
Simabuco, Fernando Moreira
[1]
Total Authors: 11
|
| Affiliation: | [1] Univ Estadual Campinas, UNICAMP, Sch Appl Sci FCA, Multidisciplinary Lab Food & Hlth LabMAS, Limeira - Brazil
[2] Univ Estadual Campinas, UNICAMP, Sch Pharmaceut Sci FCF, Lab Signal Mech, Campinas - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Phytotherapy Research; v. 35, n. 11 SEP 2021. |
| Web of Science Citations: | 1 |
| Abstract | |
Cervical cancer is the fourth leading cause of cancer mortality in women worldwide. Beetroot (Beta vulgaris L.) has bioactive compounds that can inhibit the progression of different types of cancer. To analyze the antiproliferative effects of beet leaf and root extracts, we performed MTT, clonogenic survival, cell cycle analysis, Annexin/PI labeling, and western blotting. Here, we report that 10 and 100 mu g/ml of root and leaf extracts decreased cell viability and potentiated rapamycin and cisplatin effects while decreased the number of large colonies, especially at 10 mu g/ml (293.6 of control vs. 200.0 of leaf extract, p = .0059; 138.6 of root extract, p = .0002). After 48 hr, 100 mu g/ml of both extracts led to increased sub-G1 and G0/G1 populations. In accordance, 100 mu g/ml of root extract induced early apoptosis (mean = 0.64 control vs. 1.56 root; p = .048) and decreased cell size (p < .0001). Both extracts decreased phosphorylation and expression of mechanistic Target of Rapamycin (mTOR) signaling, especially by inhibiting ribosomal protein S6 (S6) phosphorylation, increasing cleaved poly(ADP-ribose) polysomerase 1 (PARP1) and Bcl-2-like protein 11 (BIM), and decreasing cyclin D1 expression, which regulates cell cycle progression. Here, we demonstrate that beetroot and leaf extracts could be an efficient strategy against cervical cancer. (AU) | |
| FAPESP's process: | 18/14818-9 - Study of molecular targets important for the control of cancer metabolism: the mTOR/S6K pathway as a central role |
| Grantee: | Fernando Moreira Simabuco |
| Support Opportunities: | Research Grants - Young Investigators Grants - Phase 2 |
| FAPESP's process: | 16/06457-0 - Leaves and stalks of beet (Beta vulgaris L.): characterization and effects of the antioxidant capacity in obese and dyslipidemic |
| Grantee: | Rosângela Maria Neves Bezerra |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 19/25582-9 - Study of the role of Nrf2 protein in metabolism regulation and redox control in response to natural food-derived compounds in Prostate Cancer cells |
| Grantee: | Mariana Camargo Silva Mancini |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 15/00311-1 - Functional characterization of interacting proteins of different S6Ks isoforms |
| Grantee: | Isadora Carolina Betim Pavan |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 19/00607-9 - Characterization of molecular targets involved in chemotherapeutic resistance and tumor metabolism of lung cancer cells |
| Grantee: | Ana Paula Morelli |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 19/13848-4 - Evaluation of natural compounds from beetroot against cervical cancer cells HeLa |
| Grantee: | Stefhani Andrioli Romero |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |