Taurine supplementation associated with exercise increases mitochondrial activity and fatty acid oxidation gene expression in the subcutaneous white adipose tissue of obese women

Author(s):Show less -

De Carvalho, Flavia Giolo ^[1] ; Cunha Brandao, Camila Fernanda ^{[2, 3]} ; Batitucci, Gabriela ^[4] ; Souza, Anderson de Oliveira ^[5] ; Ferrari, Gustavo Duarte ^[5] ; Alberici, Luciane Carla ^[5] ; Munoz, Vitor Rosetto ^[6] ; Pauli, Jose Rodrigo ^[6] ; De Moura, Leandro Pereira ^[6] ; Ropelle, Eduardo Rochete ^[6] ; Ramos da Silva, Adelino Sanchez ^[1] ; Morandi Junqueira-Franco, Marcia Varella ^[2] ; Marchini, Julio Sergio ^[2] ; de Freitas, Ellen Cristini ^{[1, 4]}

Affiliation

^[1] Univ Sao Paulo EEFERP USP, Sch Phys Educ & Sport Ribeirao Preto, Bandeirantes Ave 3900, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo FMRP USP, Ribeirao Preto Med Sch, Internal Med Dept, Ribeirao Preto, SP - Brazil

^[3] State Univ Minas Gerais UEMG, Divinopolis, MG - Brazil

^[4] State Univ Sao Paulo FCF UNESP, Sch Pharmaceut Sci Araraquara, Dept Food & Nutr, Araraquara, SP - Brazil

^[5] Univ Sao Paulo FCFRP USP, Fac Pharmaceut Sci Ribeirao Preto, Dept BioMol Sci, Ribeirao Preto, SP - Brazil

^[6] Univ Campinas FCA UNICAMP, Lab Mol Biol Exercise, Limeira, SP - Brazil

• Document type: Journal article
• Source: Clinical Nutrition; v. 40, n. 4, p. 2180-2187, APR 2021.
• Web of Science Citations: 5

Abstract

Purpose: To evaluate the effects of taurine supplementation associated or not with chronic exercise on body composition, mitochondrial function, and expression of genes related to mitochondrial activity and lipid oxidation in the subcutaneous white adipose tissue (scWAT) of obese women. Methods: A randomized and double-blind trial was developed with 24 obese women (BMI 33.1 ? 2.9 kg/ m2, 32.9 ? 6.3 y) randomized into three groups: Taurine supplementation group (Tau, n = 8); Exercise group (Ex, n = 8); Taurine supplementation ? exercise group (TauEx, n = 8). The intervention was composed of 3 g of taurine or placebo supplementation and exercise training for eight weeks. Anthropometry, body fat composition, indirect calorimetry, scWAT biopsy for mitochondrial respiration, and gene expression related to mitochondrial activity and lipid oxidation were assessed before and after the intervention. Results: No changes were observed for the anthropometric characteristics. The Ex group presented an increased resting energy expenditure rate, and the TauEx and Ex groups presented increased lipid oxidation and a decreased respiratory quotient. Both trained groups (TauEx and Ex) demonstrated improved scWAT mitochondrial respiratory capacity. Regarding mitochondrial markers, no changes were observed for the Tau group. The TauEx group had higher expression of CIDEA, PGC1a, PRDM16, UCP1, and UCP2. The genes related to fat oxidation (ACO2 and ACOX1) were increased in the Tau and Ex groups, while only the TauEx group presented increased expression of CPT1, PPARa, PPARg, LPL, ACO1, ACO2, HSL, ACOX1, and CD36 genes. Conclusion: Taurine supplementation associated with exercise improved lipid metabolism through the modulation of genes related to mitochondrial activity and fatty acid oxidation, suggesting a browning effect in the scWAT of obese women. (c) 2020 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved. (AU)

• FAPESP's process: 17/08036-5 - Effects of taurine supplementation associated or not to exercise on mitochondrial function, energy expenditure and inflammatory profile in obese women
• Grantee: Flávia Giolo de Carvalho
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/10080-2 - Mitochondrial energy metabolism, inflammation and oxidative stress in obese women submitted to taurine supplementation and physical training
• Grantee: Ellen Cristini de Freitas
• Support Opportunities: Regular Research Grants