ovalent Immobilization of Chondrostereum purpureum Endopolygalacturonase on Ferromagnetic Nanoparticles: Catalytic Properties and Biotechnological Applicatio

Author(s)

Carli, Sibeli ^[1] ; Salgado, Jose Carlos Santos ^[1] ; Meleiro, Luana Parras ^[1] ; Ward, Richard John ^[1]

Affiliation

^[1] Univ Sao Paulo, Ciencias E Letras Ribeirao Preto, Dept Quim, Fac Filosofia, BR-14040901 Ribeirao Preto - Brazil

• Document type: Journal article
• Source: Applied Biochemistry and Biotechnology; v. 194, n. 2 SEP 2021.
• Web of Science Citations: 0

Abstract

Pectinases are widely used in a variety of industrial processes. However, their application is limited by low catalytic processivity, reduced stability, high cost, and poor re-use compatibility. These drawbacks may be overcome by enzyme immobilization with ferromagnetic nanoparticles, which are easily recovered by a magnetic field. In this work, an endopolygalacturonase from Chondrostereum purpureum (EndoPG(Cp)) expressed in Pichia pastoris was immobilized on glutaraldehyde-activated chitosan ferromagnetic nanoparticles (EndoPG(Cp)-MNP) and used to supplement a commercial enzyme cocktail. No significant differences in biochemical and kinetic properties were observed between EndoPG(Cp)-MNP and EndoPG(Cp), although the EndoPG(Cp)-MNP showed slightly increased thermostability. Cocktail supplementation with EndoPG(Cp)-MNP increased reducing sugar release from orange wastes by 1.8-fold and showed a synergistic effect as compared to the free enzyme. Furthermore, EndoPG(Cp)-MNP retained 65% of the initial activity after 7 cycles of re-use. These properties suggest that EndoPG(Cp)-MNP may find applications in the processing of pectin-rich agroindustrial residues. (AU)

• FAPESP's process: 19/17958-9 - Thermostability modulation of a ²-glucosidase stimulated by glucose and xylose using site directed glycosylation: study of glycosylation pattern by LC-MS/MS
• Grantee: Luana Parras Meleiro Garcia
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 14/50884-5 - INCT 2014: National Institute of Science and Technology of Bioethanol
• Grantee: Marcos Silveira Buckeridge
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/13734-3 - Engineering of an endopolygalacturonase by insertion of different Carbohydrate Binding Modules (CBMs): chimerogenesis and proximity effects
• Grantee: Sibeli de Carli
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/21989-7 - Bioprospection of low molecular weight fungal enzymes involved in plant cell wall degradation
• Grantee: José Carlos dos Santos Salgado
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 16/17582-0 - Thermostability modulation of a ß-glucosidase stimulated by glucose and xylose using site directed glycosylation
• Grantee: Luana Parras Meleiro Garcia
• Support Opportunities: Scholarships in Brazil - Post-Doctoral