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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Systems Biology Applied to the Study of Papaya Fruit Ripening: The Influence of Ethylene on Pulp Softening

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Author(s):
Soares, Caroline Giacomelli [1, 2] ; do Prado, Samira Bernardino Ramos [1, 2] ; Andrade, Sonia C. S. [3] ; Fabi, Joao Paulo [1, 2, 4]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Dept Food Sci & Expt Nutr, Sch Pharmaceut Sci, BR-05508000 Sao Paulo - Brazil
[2] Sao Paulo Res Fdn, Food Res Ctr FoRC, CEPID FAPESP Res Innovat & Disseminat Ctr, BR-05508080 Sao Paulo - Brazil
[3] Univ Sao Paulo, Dept Genet & Biol Evolut, Inst Biociencias, BR-05508060 Sao Paulo - Brazil
[4] Univ Sao Paulo, Food & Nutr Res Ctr NAPAN, BR-05508060 Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: CELLS; v. 10, n. 9 SEP 2021.
Web of Science Citations: 0
Abstract

Papaya is a fleshy fruit that undergoes fast ethylene-induced modifications. The fruit becomes edible, but the fast pulp softening is the main factor that limits the post-harvest period. Papaya fast pulp softening occurs due to cell wall disassembling coordinated by ethylene triggering that massively expresses pectinases. In this work, RNA-seq analysis of ethylene-treated and non-treated papayas enabled a wide transcriptome overview that indicated the role of ethylene during ripening at the gene expression level. Several families of transcription factors (AP2/ERF, NAC, and MADS-box) were differentially expressed. ACO, ACS, and SAM-Mtase genes were upregulated, indicating a high rate of ethylene biosynthesis after ethylene treatment. The correlation among gene expression and physiological data demonstrated ethylene treatment can indeed simulate ripening, and regulation of changes in fruit color, aroma, and flavor could be attributed to the coordinated expression of several related genes. Especially about pulp firmness, the identification of 157 expressed genes related to cell wall metabolism demonstrated that pulp softening is accomplished by a coordinated action of several different cell wall-related enzymes. The mechanism is different from other commercially important fruits, such as strawberry, tomato, kiwifruit, and apple. The observed behavior of this new transcriptomic data confirms ethylene triggering is the main event that elicits fast pulp softening in papayas. (AU)

FAPESP's process: 12/23970-2 - Biological changes of papaya pectins with possible benefits to human health
Grantee:Joao Paulo Fabi
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 13/07914-8 - FoRC - Food Research Center
Grantee:Bernadette Dora Gombossy de Melo Franco
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 19/11816-8 - Effect of papaya modified fibers on three-dimensional co-culture of human colon cancer cells and on in vivo model of rats with chemically induced colon carcinogenesis
Grantee:Joao Paulo Fabi
Support Opportunities: Regular Research Grants