Beetroot and leaf extracts present protective effects against prostate cancer cells, inhibiting cell proliferation, migration, and growth signaling pathways

Author(s):Show less -

Silva Mancini, Mariana Camargo ^[1] ; Saboia Ponte, Luis Gustavo ^[1] ; Rocha Silva, Cayo Henrique ^[1] ; Fagundes, Isabella ^[1] ; Betim Pavan, Isadora Carolina ^{[1, 2]} ; Romeiro, Stefhani Andrioli ^[1] ; Salvino da Silva, Luiz Guilherme ^[1] ; Morelli, Ana Paula ^[1] ; Rostagno, Mauricio Ariel ^[1] ; Simabuco, Fernando Moreira ^[1] ; Neves Bezerra, Rosangela Maria ^[1]

Affiliation

^[1] Univ Estadual Campinas, Sch Appl Sci FCA, Multidisciplinary Lab Food & Hlth LabMAS, UNICAMP, Limeira - Brazil

^[2] Univ Estadual Campinas, Sch Pharmaceut Sci FCF, Lab Signal Mech, UNICAMP, Campinas, SP - Brazil

• Document type: Journal article
• Source: Phytotherapy Research; v. 35, n. 9, p. 5241-5258, SEP 2021.
• Web of Science Citations: 1

Abstract

Beet (Beta vulgaris L.) has high nutritional value, containing bioactive compounds such as betalains and flavonoids. Scientific evidence points to the use of these natural compounds in the treatment of several types of cancer, such as prostate cancer, one of the main causes of morbidity and mortality in men. Here, we compared beet roots and leaves extracts, and their main compounds, apigenin, and betanin, respectively, in DU-145 and PC-3 prostate cancer cell lines. Both cells presented the proliferation decreased for beetroot and beet leaves extracts. The apigenin treatment also reduced the proliferation of both cell lines. Regarding cell migration, beet leaves extract was able to decrease the scratch area in both cell lines, whereas apigenin affected only PC-3 cells' migration. In colony formation assay, both extracts were effective in reducing the number of colonies formed. Besides, the beet leaves extracts and apigenin presented strong inhibition of growth-related signaling pathways in both cell lines, and the beetroot extract and betanin presented effects only in DU-145 cells. Furthermore, the extracts and isolated compounds were able to reduce the levels of apoptotic and cell cycle proteins. This study reveals that beet extracts have important anti-cancer effects against prostate cancer cells. (AU)

• FAPESP's process: 19/13848-4 - Evaluation of natural compounds from beetroot against cervical cancer cells HeLa
• Grantee: Stefhani Andrioli Romero
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 18/14818-9 - Study of molecular targets important for the control of cancer metabolism: the mTOR/S6K pathway as a central role
• Grantee: Fernando Moreira Simabuco
• Support Opportunities: Research Grants - Young Investigators Grants - Phase 2
• FAPESP's process: 16/06457-0 - Leaves and stalks of beet (Beta vulgaris L.): characterization and effects of the antioxidant capacity in obese and dyslipidemic
• Grantee: Rosângela Maria Neves Bezerra
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/00311-1 - Functional characterization of interacting proteins of different S6Ks isoforms
• Grantee: Isadora Carolina Betim Pavan
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 19/25582-9 - Study of the role of Nrf2 protein in metabolism regulation and redox control in response to natural food-derived compounds in Prostate Cancer cells
• Grantee: Mariana Camargo Silva Mancini
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 19/00607-9 - Characterization of molecular targets involved in chemotherapeutic resistance and tumor metabolism of lung cancer cells
• Grantee: Ana Paula Morelli
• Support Opportunities: Scholarships in Brazil - Master