Effects of photobiomodulation on glucose homeostasis and morphometric parameters in pancreatic islets of diabetic mice

Author(s)

Bonifacio, Mirian ^[1] ; Benfato, Izabelle Dias ^{[2, 3]} ; de Almeida Cruz, Matheus ^[4] ; de Sales, Daniele Correia ^[2] ; Pandolfo, Isabella Liba ^[1] ; Quintana, Hananiah Tardivo ^[2] ; Carvalho, Carolina Prado de Franca ^[5] ; de Oliveira, Camila Aparecida Machado ^{[3, 5]} ; Renno, Ana Claudia Muniz ^[5]

Affiliation

^[1] Univ Fed Sao Paulo UNIFESP, Grad Fisioterapia, Santos, SP - Brazil

^[2] Univ Fed Sao Paulo Unifesp, Programa Posgrad Interdisciplinar Ciencias Saude, Santos, SP - Brazil

^[3] Univ Fed Sao Paulo UNIFESP, Lab Diabet Expt & Sinalizacao Celular, Rua Silva Jardim, 136 Vila Mathias, BR-11015020 Santos, SP - Brazil

^[4] Univ Fed Sao Paulo UNIFESP, Programa Posgrad Bioprod & Bioproc, Dept Biociencias, Santos, SP - Brazil

^[5] Univ Fed Sao Paulo UNIFESP, Inst Saude & Soc, Dept Biociencias, Santos, SP - Brazil

• Document type: Journal article
• Source: Lasers in Medical Science; OCT 2021.
• Web of Science Citations: 0

Abstract

High-fat diets lead to accumulation of body fat that is associated with the onset of insulin resistance and type II diabetes mellitus. On the other hand, photobiomodulation (PBM) is an electrophysical resource that interacts with cells, stimulating mitochondrial respiration, increasing ATP production, reducing key inflammatory mediators, inhibiting apoptosis, and stimulating angiogenesis. However, little is known about its therapeutic effectiveness on the development of diabetes in diet-induced obese mice. Thus, our aim was to evaluate the effect of PBM applied single point over the pancreas area on glucose homeostasis, insulin expression, and pancreatic morphometric parameters of mice submitted to high-fat diet for 12 weeks. Male mice C57BL6/J were divided into three groups: control group (C), diabetic group (D), and diabetic + PBM (D + PBM). The treatment with PBM started at 9th week and ended in the 12th week, applied 3 x /week. Body mass, fast blood glucose, and glucose and insulin tolerance were evaluated. Immunohistochemistry to detect insulin expression and pancreatic morphometry were also performed. At the end of 12th week, both groups submitted to high-fat diet showed an increase in body mass, adiposity, disturbances on glucose homeostasis, and high insulin expression when compared to the control group. However, mice treated with PBM had more discrete impairments on glucose homeostasis during the glucose tolerance test when compared to untreated D animals. Despite modest, the results were positive and encourage future investigations to explore different doses and duration of PBM to better elucidate its role in obesity-associated type 2 diabetes development. (AU)

• FAPESP's process: 19/15566-6 - Effects of low-level laser therapy in glucose homeostasis and morphometric parameters of pancreatic islet in a diabetes model induced by diet
• Grantee: Mirian Bonifacio Silva
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 17/26075-8 - Central control of spontaneous physical activity in mice submitted or not to caloric restriction during aging process
• Grantee: Izabelle Dias Benfato
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 20/00352-8 - 3D bioprinted scaffolds from biosilica of marine sponges for treatment of bone fractures and bone defects
• Grantee: Ana Claudia Muniz Renno
• Support Opportunities: Regular Research Grants