A multivariate approach to overcome chlorophyll interferences in the determination of polycyclic aromatic hydrocarbons in jambu (Acmella olerarea (L.) RK Jansen)

Author(s)

Neves, Daniela Andrade ^[1] ; Oliveira, Wellington da Silva ^[1] ; Petrarca, Mateus Henrique ^[1] ; Rodrigues, Maria Isabel ^[2] ; Godoy, Helena Teixeira ^[1]

Affiliation

^[1] Univ Campinas UNICAMP, Fac Food Engn, Dept Food Sci, BR-13083862 Campinas, SP - Brazil

^[2] Univ Campinas UNICAMP, Fac Food Engn, Dept Food Engn, BR-13083862 Campinas, SP - Brazil

• Document type: Journal article
• Source: Journal of Food Composition and Analysis; v. 104, DEC 2021.
• Web of Science Citations: 0

Abstract

A Plackett-Burman design was used to develop an extraction method for eleven polycyclic aromatic hydrocarbons (PAHs) from jambu (Acmella oleracea (L.) R.K. Jansen), a high chlorophyll matrix. For this, a QuEChERS method was used, followed by two clean-up steps: a dispersive solid-phase extraction (d-SPE) with PSA, SAX, C18, and MgSO4, and a liquid-liquid extraction with hexane. Clear extracts with a visible reduction in the chlorophyll content were reached. Gas chromatograph mass spectrometry (GC-MS) was used for quantification. The method was validated in terms of limits of detection (LOD) and quantification (LOQ), linearity, matrix effect, precision, and recovery. The incidence of benzo(a)anthracene and benzo(b)fluoranthene in jambu was observed below the LOQ. On the other hand, chrysene was quantified in concentrations varying from 1.49 to 2.76 mu g.kg(-1). For the first time, these compounds were detected in jambu. (AU)

• FAPESP's process: 14/14248-7 - Additives, aromatic amines, nitrosamines and metals in baby bottles: analytical methodologies and study of migration
• Grantee: Wellington da Silva Oliveira
• Support Opportunities: Scholarships in Brazil - Doctorate