Discrimination of Urochloa seed genotypes through image analysis: Morphological features

Author(s)

de Freitas, Marcella Nunes ^[1] ; Neves Dias, Marcos Altomani ^[1] ; Gomes-Junior, Francisco Guilhien ^[1] ; Abud, Haynna Fernandes ^[2] ; de Araujo, Lucio Borges ^[3] ; de Moraes, Thiago Finotti ^[4]

Affiliation

^[1] Univ Sao Paulo, Dept Prod Vegetal, Escola Super Agr Luiz de Queiroz, Av Padua Dias 11, Cx Postal 9, Piracicaba, SP - Brazil

^[2] Image Pesquisas Sementes & Plantas, Ave Engenheiro Humberto Monte S-N, Bloco 310, BR-60440593 Fortaleza, Ceara - Brazil

^[3] Univ Fed Uberlandia, Fac Matemat, Campus Santa Monica, Bloco 1F, Sala 1F120, BR-38408100 Uberlandia, MG - Brazil

^[4] Univ Fed Uberlandia, Lab Eletron, BR-38400902 Uberlandia, MG - Brazil

• Document type: Journal article
• Source: AGRONOMY JOURNAL; v. 113, n. 6 OCT 2021.
• Web of Science Citations: 0

Abstract

The tropical forage species seed market, including signalgrass (Urochloa), is expanding. There is demand for fast, automated methods that can be implemented for quality control of the seeds produced, with the aim of evaluating physical purity. In this respect, seed morphological features obtained by processing radiographs with the Tomato Analyzer software and of red-green-blue obtained and processed on the Groundeye device were used to test differentiation of materials of the Urochloa genus. Seeds of Urochloa brizantha (A.Rich.) R.D.Webster cvv. BRS Piata, Marandu, and Xaraes MG-5; Urochloa ruziziensis (R.Germ. \& C.M.Evrard) Crins cv. Ruziziensis; and Urochloa decumbens (Stapf) R.D.Webster cv. Basilisk were evaluated. Morphological features obtained by Tomato Analyzer allowed differentiation of Urochloa seeds at an accuracy level greater than 80% for all the materials evaluated. Seed area was one of the features that allowed this differentiation. The Groundeye system also showed high efficiency in distinguishing Urochloa seeds, except for Piata and Xaraes, which exhibited morphological similarity, and lower accuracy levels in distinguishing their seeds (<50%). Additional studies should be conducted to evaluate Urochloa seed lots from other origins to validate the use of image analysis techniques for the purpose of obtaining morphological features and to assist in determining the physical purity of the materials. (AU)

• FAPESP's process: 15/24008-6 - Development of imaging analysis techniques to assess the vigor and internal morphology of seeds
• Grantee: Francisco Guilhien Gomes Junior
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/13139-0 - Development of imaging analysis procedures to evaluate physical, chemical and physiological properties of seeds and seedlings
• Grantee: Francisco Guilhien Gomes Junior
• Support Opportunities: Research Grants - eScience and Data Science Program - Regular Program Grants