Effect of curcumin-encapsulated Pluronic(R) F-127 over duo-species biofilm of Streptococcus mutans and Candida albicans

Author(s)

Lopes dos Santos, Diego Dantas ^[1] ; Besegato, Joao Felipe ^[2] ; Gobbo de Melo, Priscila Borges ^[2] ; Oshiro Junior, Joao Augusto ^{[3, 4]} ; Chorilli, Marlus ^[3] ; Deng, Dongmei ^{[5, 6]} ; Bagnato, Vanderlei Salvador ^[7] ; de Souza Rastelli, Alessandra Nara ^[2]

Affiliation

^[1] Sao Paulo State Univ UNESP, Sch Dent, Dept Dent Mat & Prosthodont, BR-14801903 Araraquara, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Dent, Dept Restorat Dent, 1680 Humaita St, MailBox 331, BR-14801903 Araraquara, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Drugs & Med, BR-14800903 Araraquara, SP - Brazil

^[4] State Univ Paraiba UEPB, Grad Program Pharceut Sci, BR-58429500 Campina Grande, Paraiba - Brazil

^[5] Vrije Univ Amsterdam, Amsterdam - Netherlands

^[6] Univ Amsterdam, Acad Ctr Dent Amsterdam ACTA, Dept Prevent Dent, Amsterdam - Netherlands

^[7] Univ Sao Paulo, Phys Inst Sao Carlos IFSC, Dept Phys & Mat Sci, BR-13566590 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: Lasers in Medical Science; OCT 2021.
• Web of Science Citations: 0

Abstract

To assess the effect of curcumin-encapsulated Pluronic(R) F-127 (Cur-Plu) during antimicrobial photodynamic therapy (aPDT) over duo-species biofilm of Streptococcus mutans and Candida albicans. Thermal analysis, optical absorption, and fluorescence spectroscopy were evaluated. Minimum inhibitory concentration (MIC) and minimum bactericidal/fungal concentration were obtained. The biofilms were cultured for 48 h at 37 degrees C and treated according to the groups: P + M + L + (photosensitizer encapsulated with Pluronic(R) F-127 + light); P + D + L + (photosensitizer incorporated in 1% DMSO + light); P - M + L + (no Pluronic(R) F-127 + light); P - D + L + (1% DMSO + light); P - L + (Milli-Q water + light); P + M + L - (photosensitizer encapsulated with Pluronic(R) F-127 no light); P + D + L - (photosensitizer in 1% DMSO, no light); P - M + L - (Pluronic(R) F-127 no light); P - D + L - (1% DMSO, no light); P - L - (Milli-Q water, no light; negative control group); CHX (0.2% chlorhexidine, positive control group); and NYS (Nystatin). Dark incubation of 5 min was used. The groups that received aPDT were irradiated by blue LED (460 nm, 15 J/cm(2)). Cell viability of the biofilms was performed by colony-forming units (CFU/mL) and confocal microscopy. Two-way ANOVA followed by Tukey's post hoc test was used at a significance level of 5%. P + D + L + and P + M + L + groups exhibited better log-reduction for both Candida albicans and Streptococcus mutans biofilms than P - M + L + , P - L + , and P - D + L + experimental groups. Furthermore, P + M + L + and P + D + L + showed greater reduction for Candida albicans than for Streptococcus mutans. aPDT mediated by Cur-Plu can be a potential strategy for biofilm control against duo-species biofilm of Streptococcus mutans and Candida albicans. (AU)

• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 17/17839-4 - Photodinamic inactivation using curcumin conjugated to pluronic ®F-127 on Streptococcus mutans and Candida albicans biofilm
• Grantee: Diego Dantas Lopes dos Santos
• Support Opportunities: Scholarships in Brazil - Master